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Received April 22, 2008
Accepted July 10, 2008
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Enhanced oral absorption of salmon calcitonin-encapsulated PLGA nanoparticles by adding organic substances
Tae-Sung Jung
Byung-Soo Kwon1
Hea-Eun Lee1
Ah-Young Kim1
Min-Jeong Lee1
Cho-Rong Park1
Ho-Kyung Kang
Young-Deug Kim2
Sang-Kil Lee1
Jae-Seon Kang1
Guang-Jin Choi1†
Research Center, Cell-Medicine Corp., Gimhae, Gyeongnam 621-749, Korea 1Dept. of Smart Food & Drugs and Dept. of Pharmaceutical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Korea 2Department of pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, Korea
pegchoi@inje.ac.kr
Korean Journal of Chemical Engineering, January 2009, 26(1), 131-135(5), 10.1007/s11814-009-0020-2
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Abstract
Two organic compounds with potential absorption enhancing effects, bile acids and transferrin, were examined by the gastro-intestinal (GI) absorption of therapeutic salmon calcitonin (sCT) as encapsulated by poly(lactide-co-glycolide) (PLGA) for the treatment of osteoporosis. The sCT-loaded PLGA nanocapsules were prepared by O/W emulsification approach. Either additive of a designated content was mixed with sCT dissolved in methanol. For bile acids, their content (0-7.5 mg to sCT 6 mg) was observed to have a substantial effect both on the emulsification_x000D_
process and the encapsulation efficiency. When 1.5 mg of bile acids was added, sCT-loaded PLGA nanocapsules of about 700 nm in diameter and with a fairly high encapsulation efficiency greater than 35% were produced. Accordingly, this formulation gave the most significant hypocalcemic effect in an in vivo experiment with SD rats. On the other hand, a too high bile acids loading resulted in a poor encapsulation efficiency of less than 7%. Two principal roles of_x000D_
bile acids were proposed: emulsifying agent and absorption enhancer. Transferrin, a human glycoprotein of 80 kDa molecular weight, turned out to have potential as absorption enhancer as well.
Keywords
References
Langer R, Science, 249, 1527 (1990)
Reis CP, Neufeld RJ, Ribeiro AJ, Veiga F, Nanomedicine, 2, 8 (2006)
Reis CP, Neufeld RJ, Ribeiro AJ, Veiga F, Nanomedicine, 2, 53 (2006)
Garcia-Fuentes M, Prego C, Torres D, Alonso MJ, Euro. J. Pharm. Sci., 25, 133 (2005)
Astete CE, Sabliov CM, J. Biomater. Sci. - Polym. Ed., 17, 247 (2006)
Nah JW, Jeong YI, Koh JJ, Korean J. Chem. Eng., 17(2), 230 (2000)
Mikos AG, Mathiowitz E, Langer R, Peppas NA, Colloid Interface Sci., 143, 366 (1991)
Eldridge JH, Hammond CJ, Muelbroek JA, Staas JK, Gilley RM, Tice TR, J. Control. Release, 11, 205 (1990)
Tobio M, Sanchez A, Vila A, Soriano I, Evora C, Vila-Jato JL, Alonso MJ, Colloid. Surf. B: Biointerf., 18, 315 (2000)
Vila A, Tobio M, Calvo P, Alonso MJ, J. Control. Release, 78, 15 (2002)
Prego C, Garcia M, Torres D, Alonso MJ, J. Control. Release, 101, 151 (2005)
Yamamoto H, Kuno Y, Sugimoto S, Takeuchi H, Kawashima Y, J. Control. Release, 102, 373 (2005)
Prego C, Torres D, Fernandez-Megia E, Novoa-Carballal R, Quinoa E, Alonso MJ, J. Control. Release, 111, 299 (2006)
Youn YS, Jung JY, Oh SH, Yoo SD, Lee KC, J. Control. Release, 114, 334 (2006)
Song KH, Chung SJ, Shim CK, J. Control. Release, 106, 298 (2005)
Yamamoto A, Iseki T, Ochi-Sugiyama M, Okada N, Fujita T, Muranishi S, J. Control. Release, 76, 363 (2001)
Fetih G, Habib F, Okada N, Fujita T, Attia M, Yamamoto A, J. Control. Release, 106, 287 (2005)
Imai T, Sakai M, Ohtake H, Azuma H, Otagiri M, Int. J. Pharmaceutics, 294, 11 (2005)
Takatsuka S, Morita T, Koguchi A, Horikin Y, Yamahara H, Yoshino H, Int. J. Pharmaceutics, 316, 124 (2006)
Matsuyama T, Morita T, Horikiri Y, Yamahara H, Yoshino H, J. Control. Release, 110, 347 (2006)
Matsuyama T, Morita T, Horikiri Y, Yamahara H, Yoshino H, J. Control. Release, 120, 88 (2007)
Jang JY, Kwon BS, Lee HE, Kim DH, Kang HK, Kang JS, Lee S, Choi GJ, J. Ind. Eng. Chem., 13(6), 1043 (2007)
Jung T, Kamm W, Breitenbach A, Kaiserling E, Xiao JX, Kittel T, Eur. J. Pharmaceutics & Biopharmaceutics, 50, 147 (2000)
Ritchie RF, Palomaki GE, Neveux LM, Navolotskaia O, Ledue TB, Craig WY, J. Clin. Lab. Anal., 13, 273 (1999)
Reis CP, Neufeld RJ, Ribeiro AJ, Veiga F, Nanomedicine, 2, 8 (2006)
Reis CP, Neufeld RJ, Ribeiro AJ, Veiga F, Nanomedicine, 2, 53 (2006)
Garcia-Fuentes M, Prego C, Torres D, Alonso MJ, Euro. J. Pharm. Sci., 25, 133 (2005)
Astete CE, Sabliov CM, J. Biomater. Sci. - Polym. Ed., 17, 247 (2006)
Nah JW, Jeong YI, Koh JJ, Korean J. Chem. Eng., 17(2), 230 (2000)
Mikos AG, Mathiowitz E, Langer R, Peppas NA, Colloid Interface Sci., 143, 366 (1991)
Eldridge JH, Hammond CJ, Muelbroek JA, Staas JK, Gilley RM, Tice TR, J. Control. Release, 11, 205 (1990)
Tobio M, Sanchez A, Vila A, Soriano I, Evora C, Vila-Jato JL, Alonso MJ, Colloid. Surf. B: Biointerf., 18, 315 (2000)
Vila A, Tobio M, Calvo P, Alonso MJ, J. Control. Release, 78, 15 (2002)
Prego C, Garcia M, Torres D, Alonso MJ, J. Control. Release, 101, 151 (2005)
Yamamoto H, Kuno Y, Sugimoto S, Takeuchi H, Kawashima Y, J. Control. Release, 102, 373 (2005)
Prego C, Torres D, Fernandez-Megia E, Novoa-Carballal R, Quinoa E, Alonso MJ, J. Control. Release, 111, 299 (2006)
Youn YS, Jung JY, Oh SH, Yoo SD, Lee KC, J. Control. Release, 114, 334 (2006)
Song KH, Chung SJ, Shim CK, J. Control. Release, 106, 298 (2005)
Yamamoto A, Iseki T, Ochi-Sugiyama M, Okada N, Fujita T, Muranishi S, J. Control. Release, 76, 363 (2001)
Fetih G, Habib F, Okada N, Fujita T, Attia M, Yamamoto A, J. Control. Release, 106, 287 (2005)
Imai T, Sakai M, Ohtake H, Azuma H, Otagiri M, Int. J. Pharmaceutics, 294, 11 (2005)
Takatsuka S, Morita T, Koguchi A, Horikin Y, Yamahara H, Yoshino H, Int. J. Pharmaceutics, 316, 124 (2006)
Matsuyama T, Morita T, Horikiri Y, Yamahara H, Yoshino H, J. Control. Release, 110, 347 (2006)
Matsuyama T, Morita T, Horikiri Y, Yamahara H, Yoshino H, J. Control. Release, 120, 88 (2007)
Jang JY, Kwon BS, Lee HE, Kim DH, Kang HK, Kang JS, Lee S, Choi GJ, J. Ind. Eng. Chem., 13(6), 1043 (2007)
Jung T, Kamm W, Breitenbach A, Kaiserling E, Xiao JX, Kittel T, Eur. J. Pharmaceutics & Biopharmaceutics, 50, 147 (2000)
Ritchie RF, Palomaki GE, Neveux LM, Navolotskaia O, Ledue TB, Craig WY, J. Clin. Lab. Anal., 13, 273 (1999)
