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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received April 29, 2008
Accepted July 29, 2008

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Properties of bacterial cellulose produced in a pilot-scale spherical type bubble column bioreactor

Chang Nam Choi Hyo Jeong Song¹ Myong Jun Kim¹ Mi Hwa Chang Seong Jun Kim^1†

Department of Textile Engineering, Chonnam National University, Gwangju 500-757, Korea ¹Department of Civil, Earth and Environmental Engineering, Chonnam National University, Gwangju 500-757, Korea

seongjun@chonnam.ac.kr

Korean Journal of Chemical Engineering, January 2009, 26(1), 136-140(5), 10.1007/s11814-009-0021-1

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Abstract

The saccharogenic liquid obtained by the enzymatic saccharification of food wastes was used as a medium for production of bacterial cellulose (BC). The enzymatic saccharification of food wastes (SFW) was carried out by the cultivation supernatant of Trichoderma inhamatum KSJ1. 5.6 g/L of BC was produced in a new modified 50 L bubble column bioreactor by Acetobacter xylinum KJ1. The productivity was similar to that of a modified 10 L bubble column bioreactor (5.8 g/L). When pure oxygen was supplied into the scaled-up culture conditions, 6.8 g/L (12% enhancement) of BC was produced, indicating a very useful method for BC mass production. The oxygen uptake rate (OUR) and qO2 (specific oxygen uptake rate) were 0.214 mg-DO/L·min and 0.257 mg-DO/g-cell·min, respectively. The physical properties, such as morphology, molecular weight, crystallinity, and tensile strength of BCs produced in static culture (A), 10 L (B) and 50 L (C) modified bubble column cultures were investigated. All BCs showed fibrils_x000D_ with highly networking structure. The number average molecular weight of BCs in A, B and C was 2,314,000, 1,878,000, and 1,765,000, respectively. All of the BCs had a form of cellulose I representing pure cellulose. The relative degree of crystallinity showed the range of 79.6-86.0%. Tensile strengths of BC sheet in A, B and C were 1.75, 1.21, and 1.19 kg/mm^(2), respectively. In conclusion, BC production by the modified bubble column culture mode of 50 L brought more_x000D_ favorable results in terms of the physical properties and its ease of scale-up.

Keywords

Saccharification of Food Wastes (SFW) Bacterial Cellulose (BC) Pilot Scale Production Modified Bubble Column Bioreactor Physical Properties

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