Articles & Issues

Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received April 29, 2008
Accepted August 3, 2008

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

All issues

Pilot-scale production of bacterial cellulose by a spherical type bubble column bioreactor using saccharified food wastes

Hyo-Jeong Song Hongxian Li¹ Jin-Ho Seo Myong-Jun Kim¹ Seong-Jun Kim^1†

Department of Urban Engineering, Chonnam National University, Daejeon 305-764, Korea ¹Department of Civil, Earth and Environmental Engineering, Chonnam National University, Gwangju 500-757, Korea

seongjun@jnu.ac.kr

Korean Journal of Chemical Engineering, January 2009, 26(1), 141-146(6), 10.1007/s11814-009-0022-0

Download PDF

Abstract

Bacterial cellulose (BC) was produced by Acetobacter xylinum KJ1 in a modified airlift-type bubble column bioreactor, which had a low shear stress and high oxygen transfer rate (kLa). Saccharified food wastes (SFW) were used as the BC production medium due to its low cost. An aeration rate of 1.2 vvm (6 L/min) was tentatively determined as the optimal aeration condition in a 10 L spherical type bubble column bioreactor, by analysis of the oxygen transfer coefficient. When 0.4% agar was added, the BC production reached 5.8 g/L, compared with 5.0 g/L in the culture without the addition of agar. The BC productivity was improved by 10% in the addition of 0.4% agar into the SFW medium. Then, by conversion of a linear velocity of 0.93 cm/sec, from the relationship between the linear velocity and oxygen transfer rate, 1.0 vvm (30 L/min) was determined as an optimal aeration condition in a 50 L spherical type bubble column reactor. Using SFW medium, with the addition of 0.4% agar, and air supplied of 1.0 vvm, 5.6 g/L BC_x000D_ was produced in the 50 L spherical type bubble column bioreactor after 3 days of cultivation, which was similar to that produced in the 10 L bioreactor. In conclusion, the addition of agar, a viscous polysaccharide, into SFW medium is effective for the production of BC, and this scale-up method is very useful for the mass production in a 50 L spherical type bubble column bioreactor by decreasing the shear stress and increasing the kLa.

Keywords

Scale-up Bacterial Cellulose Saccharified Food Wastes Spherical Type Bubble Column Bioreactor

References

Rainer J, Luiz FF, Polym. Degrad Stab., 58, 101 (1998)
Yamanake S, Watanabe K, Applications of bacterial cellulose in cellulosic polymers, In Gillbert R (ed), Cellulosic Polymers, Blends and Composites, Hanser Inc., Cincinnati, OH, USA (1995)
Cannon RE, Anderson SM, Crit. Rev. Microbiol., 17, 435 (1991)
Klemm D, Schumann D, Udhard U, Marsch S, Prog. Polym. Sci., 26, 1561 (2001)
Moon SH, Park JM, Chun HY, Kim SJ, Biotechnol. Bioprocess. Eng., 11, 26 (2006)
Brown AJ, J. Chem. Soc., 49, 432 (1886)
Chao Y, Ishida T, Sugano Y, Shoda M, Biotechnol. Bioeng., 68, 345 (2000)
Valla S, Kjosbakken J, J. Gen. Microbiol., 128, 1401 (1982)
Kim KC, Kim SW, Kim MJ, Kim SJ, Biotechnol. Bioprocess. Eng., 10, 52 (2005)
Mandel M, Sternberg D, J. Ferment. Technol., 54, 267 (1976)
Son CJ, Chung SY, Lee JE, Kim SJ, J. Appl. Microbiol. Biotechnol., 12, 722 (2002)
Hestrin S, Schramm M, Biochem. J., 56, 162 (1954)
Wise WS, J. Gen. Microbiol., 5, 166 (1951)
Thomas MW, Bhat KM, Method Enzymol., 160, 87 (1988)
Richards JW, Introduction to industrial sterilization, Academic Press, London (1968)
Onken U, Weiland P, In advances in biotechnological processes, Alan R. Liss, Inc., New York, 1, 67 (1983)
Ranade VR, Ulbrecht JJ, AIChE J., 24, 796 (1978)
Ishida T, Mitarai M, Sugano Y, Shoda M, Biotechnol. Bioeng., 83(4), 474 (2003)