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Received April 21, 2008
Accepted July 5, 2008
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Applications of silver nanoplates as colorimetric indicators of pH-induced conformational changes in cytochrome c
Department of Chemical Engineering, Kwangwoon University, Wolgye-dong, Nowon-gu, Seoul 139-701, Korea
korea1@kw.ac.kr
Korean Journal of Chemical Engineering, January 2009, 26(1), 258-260(3), 10.1007/s11814-009-0043-8
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Abstract
Silver nanoplates (AgNPs) were used as a colorimetric indicator of pH-induced conformational changes in the protein, cytochorme c (cyt c). Cyt c was covalently bound to the surface of AgNPs, and UV-vis spectroscopy was used to identify changes in the optical properties of the resulting cyt c-AgNP conjugates in colloidal solution over pH, with values ranging from 3.1 to 10.6. Transmission electron microscopy (TEM) results indicated that the morphological changes in cyt c-AgNP conjugates were dependent on pH. Moreover, pH-induced conformational changes in cyt c could be detected by visual inspection of the color changes in cyt c-AgNPs, which occurred as a result of the coupling effect of localized surface plasmon resonance (LSPR) by aggregated AgNPs.
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References
Mirkin CA, Letsinger RL, Mucic RC, Storhoff JJ, Nature, 382(6592), 607 (1996)
Choi S, Kim KS, Yeon SH, Cha JH, Lee H, Kim CJ, Yoo ID, Korean J. Chem. Eng., 24(5), 856 (2007)
Kelly KL, Coronado E, Zhao LL, Schatz GC, J. Phys. Chem., 107, 668 (2003)
Haes AJ, Zou SL, Schatz GC, Van Duyne RP, J. Phys. Chem. B, 108(22), 6961 (2004)
Elghanian R, Storhoff JJ, Mucic RC, Letsinger RL, Mirkin CA, Science, 277(5329), 1078 (1997)
Chah S, Hammond MR, Zare RN, Chem Biol., 12, 323 (2005)
Worrall JWE, Verma A, Yan H, Rotello VM, Chem. Comm., 22, 2338 (2006)
Salafsky JS, J. Chem. Phys., 125, 074701 (2006)
Jiang X, Qu X, Zhang L, Zhang Z, Jiang J, Wang E, Dong S, Biophys. Chem., 110, 203 (2004)
Chah S, Kumar CV, Hammond MR, Zare RN, Anal. Chem., 76, 2112 (2004)
Jiang X, Zeng Q, Yu A, Nanotechnology, 17, 4929 (2006)
Ferrer JC, Guillemette JG, Bogumil R, Inglis SC, Smith M, Mauk AG, J. Am. Chem. Soc., 115, 7507 (1993)
Santucci R, Bongiovanni C, Mei G, Ferri T, Polizio F, Desideri A, Biochemistry, 39, 1632 (2000)
Garrett RH, Grisham CM, Biochemistry, Harcourt, Orlando, FL (1999)
Li GT, Wang TY, Bhosale S, Zhang Y, Fuhrhop JH, Colloid Polym. Sci., 281, 1099 (2003)
Han X, Li Y, Wu S, Deng Z, Small, 4, 326 (2008)
Chen Y, Wang C, Ma Z, Su Z, Nanotechnology, 18, 325602 (2007)
Choi S, Kim KS, Yeon SH, Cha JH, Lee H, Kim CJ, Yoo ID, Korean J. Chem. Eng., 24(5), 856 (2007)
Kelly KL, Coronado E, Zhao LL, Schatz GC, J. Phys. Chem., 107, 668 (2003)
Haes AJ, Zou SL, Schatz GC, Van Duyne RP, J. Phys. Chem. B, 108(22), 6961 (2004)
Elghanian R, Storhoff JJ, Mucic RC, Letsinger RL, Mirkin CA, Science, 277(5329), 1078 (1997)
Chah S, Hammond MR, Zare RN, Chem Biol., 12, 323 (2005)
Worrall JWE, Verma A, Yan H, Rotello VM, Chem. Comm., 22, 2338 (2006)
Salafsky JS, J. Chem. Phys., 125, 074701 (2006)
Jiang X, Qu X, Zhang L, Zhang Z, Jiang J, Wang E, Dong S, Biophys. Chem., 110, 203 (2004)
Chah S, Kumar CV, Hammond MR, Zare RN, Anal. Chem., 76, 2112 (2004)
Jiang X, Zeng Q, Yu A, Nanotechnology, 17, 4929 (2006)
Ferrer JC, Guillemette JG, Bogumil R, Inglis SC, Smith M, Mauk AG, J. Am. Chem. Soc., 115, 7507 (1993)
Santucci R, Bongiovanni C, Mei G, Ferri T, Polizio F, Desideri A, Biochemistry, 39, 1632 (2000)
Garrett RH, Grisham CM, Biochemistry, Harcourt, Orlando, FL (1999)
Li GT, Wang TY, Bhosale S, Zhang Y, Fuhrhop JH, Colloid Polym. Sci., 281, 1099 (2003)
Han X, Li Y, Wu S, Deng Z, Small, 4, 326 (2008)
Chen Y, Wang C, Ma Z, Su Z, Nanotechnology, 18, 325602 (2007)
