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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received April 11, 2008
Accepted September 7, 2008

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Continuous production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate): Effects of C/N ratio and dilution rate on HB/HV ratio

Shwu-Tzy Wu^† Yi-Chun Lin Jui-Rze Too

Department of Bioindustry Technology, Da-Yeh University, Chang-Hua 51591, Taiwan, ROC

Korean Journal of Chemical Engineering, March 2009, 26(2), 411-416(6), 10.1007/s11814-009-0069-y

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Abstract

Ralstonia eutropha was cultivated in a continuous stirred fermenter with various C/N ratios (20, 30, and 40), dilution rates, and organic salt substrates (sodium propionate or sodium valerate) to explore the microbial growth and the poly(3HB-co-3HV) accumulation. When sodium propionate was used as the secondary carbon source, the HB/HV molar ratio at various C/N ratios and dilution rates did not change appreciably (approximately 90 : 10). The highest poly(3HB-co-3HV) content in biomass (41.8%) and poly(3HB-co-3HV) productivity (0.100 g/(L·h)) occurred under_x000D_ the condition with a C/N ratio of 20 and dilution rate of 0.06 h^(-1). When sodium valerate was used as the secondary carbon source, the productivity of poly(3HB-co-3HV) increased with increasing dilution rate for the C/N ratio of 30 and 40. The average HB/HV molar ratio ranged from 48 : 52 to 78 : 32. The feeding of sodium valerate promoted the accumulation of HV better than feeding sodium propionate did. This study shows that a potential strategy of manipulating by both C/N ratio and dilution rate could be used to control the HV unit fraction in poly(3HB-co-3HV) in a continuous cultivation.

Keywords

Poly(3HB-co-3HV) Continuous Cultivation Dilution Rate Ralstonia eutropha C/N Ratio Propionate Valerate

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