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Received July 7, 2008
Accepted September 7, 2008
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Fermentation process development for hyaluronic acid production by Streptococcus zooepidemicus ATCC 39920
Department of Chemical and Materials Engineering, National Kaohsiung University of Applied Sciences, No.415, Chien Kung Road, Kaohsiung 80778, Taiwan 1Department of Chemical Engineering, National Cheng Kung University, No.1, University Road, Tainan 70101, Taiwan
biochen@cc.kuas.edu.tw
Korean Journal of Chemical Engineering, March 2009, 26(2), 428-432(5), 10.1007/s11814-009-0072-3
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Abstract
The development of a fermentation strategy for hyaluronic acid (HA) production by Streptococcus zooepidemicus ATCC 39920 has been explored. The specific HA productivity (Y P/X) was affected by the medium carbon-tonitrogen (C/N) ratio rather than the specific growth rate of cells. Accordingly, HA fermentation should be performed in a balanced medium with an optimum C/N ratio of 2 : 1 in a batch culture. To improve the performance of the batch culture, the operation conditions for the fill-and-draw culture were investigated. It was found that the timing of medium_x000D_
exchange is critical for successfully performing fill-and-draw operations. Since streptococcal cells at the stationary phase might lose the capacity of HA synthesis, the displacement of the medium in a fill-and-draw culture should be started at the late exponential growth phase.
Keywords
References
Weissmann B, Meyer K, J. Am. Chem. Soc., 76, 1753 (1954)
Liesegang TJ, Survey Ophthalmol., 34, 268 (1990)
Radaeva IF, Kostina GA, Zmievskii AV, Appl. Biochem. Microbiol., 33, 111 (1997)
Lapcik, Jr. L, Lapcik L, De Smedt S, Demeester J, Chabrecek P, Chem. Rev., 8, 2663 (1998)
Chong BF, Blank LM, Mclaughlin R, Nielsen LK, Appl. Microbiol. Biotechnol., 66(4), 341 (2005)
Khan T, Park JK, Kwon JH, Korean J. Chem. Eng., 24(5), 816 (2007)
Johns MR, Goh LT, Oeggerli A, Biotechnol. Lett., 16(5), 507 (1994)
Kim JH, Yoo SJ, Oh DK, Kweon YG, Park DW, Lee CH, Gil GH, Enzyme Microb. Technol., 19(6), 440 (1996)
Armstrong DC, Cooney MJ, Johns MR, Appl. Microbiol. Biotechnol., 47(3), 309 (1997)
Armstrong DC, Johns MR, Appl. Environ. Microbiol., 63, 2759 (1997)
Hasegawa S, Nagatsuru M, Shibutani M, Yamamoto S, Hasebe S, J. Biosci. Bioeng., 88(1), 68 (1999)
Gao HJ, Du GC, Chen J, World J. Microbiol. Biotechnol., 22, 399 (2006)
Kim SJ, Park SY, Kim CW, J. Microbiol. Biotchnol., 16, 1849 (2006)
Hiruta O, Yamamura K, Takebe H, Futamura T, Iinuma K, Tanaka H, J. Ferment. Bioeng., 83(1), 79 (1997)
Patkar A, Lee DH, Seo JH, Korean J. Chem. Eng., 10(3), 146 (1993)
Cheng LC, Wu JY, Chen TL, Biochem. Eng. J., 10, 227 (2002)
Arpornwichanop A, Shomchoam N, Korean J. Chem. Eng., 24(1), 11 (2007)
Huang WC, Chen SJ, Chen TL, Biochem. Eng. J., 32, 239 (2006)
Fong Chong B, Nielsen LK, J. Biotechnol., 100, 33 (2003)
Bitter T, Muir HM, Anal. Biochem., 4, 330 (1962)
Miller GL, Anal. Chem., 31, 426 (1959)
Blank LM, McLaughlin RL, Nielsen LK, Biotechnol. Bioeng., 90, 686 (2005)
van de Rijn I, J. Bacteriol., 156, 1059 (1983)
Liesegang TJ, Survey Ophthalmol., 34, 268 (1990)
Radaeva IF, Kostina GA, Zmievskii AV, Appl. Biochem. Microbiol., 33, 111 (1997)
Lapcik, Jr. L, Lapcik L, De Smedt S, Demeester J, Chabrecek P, Chem. Rev., 8, 2663 (1998)
Chong BF, Blank LM, Mclaughlin R, Nielsen LK, Appl. Microbiol. Biotechnol., 66(4), 341 (2005)
Khan T, Park JK, Kwon JH, Korean J. Chem. Eng., 24(5), 816 (2007)
Johns MR, Goh LT, Oeggerli A, Biotechnol. Lett., 16(5), 507 (1994)
Kim JH, Yoo SJ, Oh DK, Kweon YG, Park DW, Lee CH, Gil GH, Enzyme Microb. Technol., 19(6), 440 (1996)
Armstrong DC, Cooney MJ, Johns MR, Appl. Microbiol. Biotechnol., 47(3), 309 (1997)
Armstrong DC, Johns MR, Appl. Environ. Microbiol., 63, 2759 (1997)
Hasegawa S, Nagatsuru M, Shibutani M, Yamamoto S, Hasebe S, J. Biosci. Bioeng., 88(1), 68 (1999)
Gao HJ, Du GC, Chen J, World J. Microbiol. Biotechnol., 22, 399 (2006)
Kim SJ, Park SY, Kim CW, J. Microbiol. Biotchnol., 16, 1849 (2006)
Hiruta O, Yamamura K, Takebe H, Futamura T, Iinuma K, Tanaka H, J. Ferment. Bioeng., 83(1), 79 (1997)
Patkar A, Lee DH, Seo JH, Korean J. Chem. Eng., 10(3), 146 (1993)
Cheng LC, Wu JY, Chen TL, Biochem. Eng. J., 10, 227 (2002)
Arpornwichanop A, Shomchoam N, Korean J. Chem. Eng., 24(1), 11 (2007)
Huang WC, Chen SJ, Chen TL, Biochem. Eng. J., 32, 239 (2006)
Fong Chong B, Nielsen LK, J. Biotechnol., 100, 33 (2003)
Bitter T, Muir HM, Anal. Biochem., 4, 330 (1962)
Miller GL, Anal. Chem., 31, 426 (1959)
Blank LM, McLaughlin RL, Nielsen LK, Biotechnol. Bioeng., 90, 686 (2005)
van de Rijn I, J. Bacteriol., 156, 1059 (1983)
