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Received July 19, 2008
Accepted September 10, 2008
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Enhancing the sporulation of Streptomyces kasugaensis by culture optimization
Department of Biological Engineering, Inha University, Incheon 402-751, Korea
ekkim@inha.ac.kr
Korean Journal of Chemical Engineering, March 2009, 26(2), 438-443(6), 10.1007/s11814-009-0074-1
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Abstract
To be an effective microbial biocide, Streptomyces kasugaensis should be converted into spore during cultivation process for successful long-term storage. By statistical design methods, culture conditions including medium components and operating parameters were optimized and more than 100 times increase in spore yield was achieved. Addition of spent culture fluid (100 ppm), EDTA (30 ppm), mycophenolic acid (32 ppm) with combination of pH upshock (5.5 to 8.5) increased total viable cell and spore conversion rate, resulting in 1.6×10^(7) (spore/mL) in 5 days of culture in a fermenter. This result provides a practical method for obtaining high spore number for commercial production of Streptomyces kasugaensis as a microbial pesticide.
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Biro S, Bekesi I, Vitalis S, Szabo G, Eur. J. Biochem., 103, 359 (1980)
Slieman TA, Nicholson WL, Appl. Environ. Microbiol., 67, 1274 (2001)
Flardh K, Cur. Opin. Microbiol., 6, 564 (2003)
Yang YK, Morikawa M, Shimizu H, Shioya S, Suga K, Nihira T, Yamada Y, J. Ferment. Bioeng., 81(1), 7 (1996)
Miguelez EM, Rueda B, Hardisson C, Manzanal MB, FEMS Microbiol. Lett., 157, 103 (1997)
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Beres DL, Hawkins DM, Ecol. Mod., 141, 171 (2001)
Kucuk O, Korean J. Chem. Eng., 23(1), 21 (2006)
Li Y, Xing J, Li W, Xiong X, Li X, Liu H, Korean J. Chem. Eng., 24(5), 781 (2007)
Manoja RL, Serror P, Wong KW, Sonenshein AL, Gene Dev., 15, 1093 (2001)
Itoh M, Penyige A, Okamoto S, Ochi K, FEMS Microbiol. Lett., 135, 311 (1996)
Ochi K, J. Bacteriol., 169, 3608 (1987)
Vidwans SJ, Ireton K, Grossman A, J. Bacteriol., 177, 3308 (1995)
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Tough AJ, Prosser JI, Microbiology, 142, 639 (1996)
Quiros LM, Salas JA, FEMS Microbiol. Lett., 141, 245 (1996)
Novella IS, Sanchez J, Res. Microbiol., 146, 721 (1995)
