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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received July 19, 2008
Accepted September 24, 2008

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Optimal culture condition for the production of phenyalanine ammonia lyase from E. coli

Jian-dong Cui^† Yan Li

College of Bioscience and Bioengineering, Hebei University of Science and Technology, 70 YuHua East Road,Shijiazhuang 050018, P. R. China

cjd007cn@163.com

Korean Journal of Chemical Engineering, March 2009, 26(2), 444-448(5), 10.1007/s11814-009-0075-0

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Abstract

The effects of culturing conditions on phenylalanine ammonia lyase production by a recombinant E. coli strain were investigated by using a controlled fed-batch fermentation system. In a 5 L fermentor, the optimal composition of the batch medium was 2% glucose, 1% yeast extract, 0.7% K2HPO4, 0.8% KH2PO4, 0.5% (NH4)2SO4, 0.1% MgSO4·7H2O. The optimal feed glucose solution was 50%. Glucose concentration and pH of the culture broth were maintained at about 2.0 g/L and 7.0 during the fed-batch phase, respectively. Following 24-h cultivation, 0.2 mmol/L isopropyl-β-D-thiogalactopyranoside (IPTG) was added and temperature was shifted from 37 ℃ to 42 ℃ to induce pal gene expression. Under optimal conditions, a high productivity of 300 U/g could be achieved after 48 h culture, and a cell density of OD600 about 82 was obtained at 52 h culture at 500 r/m stirrer speed and 1 vvm, respectively.

Keywords

Activity Culturing Conditions Fed-batch Phenylalanine Ammonia Lyase Recombinant Escherichia coli

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