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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received July 31, 2008
Accepted August 27, 2008

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of pH on fractional precipitation for pre-purification of paclitaxel from plant cell cultures

Seul-Gi Kim Jin-Hyun Kim^†

Department of Chemical Engineering, Kongju National University, 182, Shinkwan-Dong, Kongju, Chungnam 314-701, Korea

Korean Journal of Chemical Engineering, March 2009, 26(2), 449-452(4), 10.1007/s11814-009-0076-z

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Abstract

Fractional precipitation is a simple, efficient method for pre-purifying paclitaxel from plant cell cultures of Taxus chinensis. The pH, a key process variable in fractional precipitation, was optimized in terms of the yield and purity of paclitaxel with a fixed methanol concentration in water (61.5%, v/v), paclitaxel content in the crude extract (0.5%, w/v), and storage time (14 hr). The maximum yield (~99%) and purity (~84.4%) of paclitaxel were obtained at pH 8.0 with potassium phosphate buffer. The precipitate from fractional precipitation was an amorphous state paclitaxel by analysis of XRD and SEM. Also, the particle size distribution of dried precipitate was a range of approximately 119-411 μm. The use of fractional precipitation in the pre-purification process allowed for rapid and efficient separation of paclitaxel from interfering compounds, and dramatically increased the yield and purity of the crude paclitaxel for subsequent purification steps.

Keywords

Paclitaxel Fractional Precipitation pH Optimization Pre-purification

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