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Received September 26, 2008
Accepted December 23, 2008
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Effects of moderate pressure on premeability and viability of Saccharomyces cerevisiae cells
Key Laboratory of Industry Microbiology, Ministry of Education, Tianjin University of Science and Technology, 29 Thirteenth Street, Tai Da Development Area, Tianjin 300457, P. R. China 1Hebei Fermentation Engineering Research Center, College of Bioscience and Bioengineering, Hebei University of Scienceand Technology, 70 Yuhua East Road, Shijiazhang 050018, P. R. China
jiashiru@tust.edu.cn
Korean Journal of Chemical Engineering, May 2009, 26(3), 731-735(5), 10.1007/s11814-009-0122-x
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Abstract
With CO2 and N2 as the pressure media, the effects of the moderate pressure (0.1-1.0MPa) and the holding time on the conductivities of the cell suspension of Saccharomyces cerevisiae CICC1447 and Saccharomyces cerevisiae CICC1339, as well as the absorbances of the supernatant (after centrifuged) at 280 nm (A280) and 260 nm (A260) were determined. The membrane permeability of Saccharomyces cerevisiae CICC1447 increased significantly and the cell leakage was aggravated with the pressure increase. For Saccharomyces cerevisiae CICC1339, the conductivity of the cell suspension, A280 and A260 of the supernatant fluctuated with the pressure increase; as a whole, they increased with pressure. Different from high pressure, a moderate pressure not only remarkably improved the permeability of the yeast_x000D_
cell membrane, but also kept yeast cell viability; moreover, the integrity of the yeast cell membrane could be maintained.
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