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Received November 11, 2008
Accepted December 21, 2008
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Optimization of culture media for Bacillus species by statistical experimental design methods
Department of Biological Engineering, Inha University, Incheon 402-751, Korea
ekkim@inha.ac.kr
Korean Journal of Chemical Engineering, May 2009, 26(3), 754-759(6), 10.1007/s11814-009-0126-6
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Abstract
The medium compositions of Bacillus species (three strains) were optimized sequentially through two statistical experimental designs, Plackett-Burman and the Box-Behnken, which were determined by representative threefactor response-surface methodology. pH and DO control was found to enhance the viable cell number of Bacilli. Most strains grew well in the optimum condition and reached nearly up to of 5×10^(9) CFU/mL. Additionally, when three of the Bacilli were cultivated in the optimized medium with pH and DO control, the viable cell number increased 3.6 times in B. subtilis, 64.1 times in B. licheniformis and 8.3 times in B. coagulans, respectively, in comparison with the original industrial medium.
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References
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Veld HI, JHJ, Havenaar R, J. Chem. Technol. Biotechnol., 51, 562 (1991)
McCann T, Egan T, Weber GH, J. Food Protect, 59, 41 (1996)
Liu BL, Tzeng YM, Biopro. Eng., 18, 413 (1998)
Chan L, Bai J, Cai Z, Ouyang F, J. Biotechnol., 93, 27 (2002)
Krishnan S, Prapulla SG, Rajalakshmi D, Misra MC, Karanth NG, Biopro. Eng., 19, 61 (1998)
Pham PL, Taillandier P, Delmas M, Strehaiano P, World J. Microbiol. Biotechnol., 14, 185 (1998)
Beres DL, Hawkins DM, Ecol. Mod., 141, 171 (2001)
Sunitha K, Lee JK, Oh TK, Biopro. Eng., 21, 477 (1999)
Park K, Reardon KF, Biotechnol. Lett., 18(6), 737 (1996)
Philippe J, Hbid C, Destain J, Razafindralambo H, Paquot M, Pauw ED, Thonart P, Biochem. Biotechnol., 77, 223 (1999)
Lee IY, Kim MK, Lee JH, Seo WT, Lee HW, Park YH, Biopro. Eng., 20, 283 (1999)
Kucuk O, Korean J. Chem. Eng., 23(1), 21 (2006)
