Articles & Issues

Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received December 21, 2008
Accepted January 12, 2009

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

All issues

Production of hydrocortisone by Absidia coerulea in moderate pressure bioconversion system

Shi Ru Jia^† Jian Dong Cui¹

Key Laboratory of Industry Microbiology, Ministry of Education, Tianjin University of Science and Technology, 29 Thirteenth Street, Tai Da Development Area, Tianjin 300457, P. R. China ¹Hebei Fermentation Engineering Research Center, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhang 050018, P. R. China

jiashiru@tust.edu.cn

Korean Journal of Chemical Engineering, July 2009, 26(4), 1084-1089(6), 10.1007/s11814-009-0180-0

Download PDF

Abstract

The effects of moderate pressure (0.1-2.5 MPa) on viability, cell membrane permeability and catalyzing activity of Absidia coerulea for RSA were investigated. A new method for improving the production of Hydrocortisone (HC) from 17α-hydroxypregn-4-en-3, 20-dione-21-acetate by Absidia coerulea in moderate pressure was developed. The results showed that the morphology of Abasidia coerulea mycelium was changed in moderate pressure, Absidia coerulea mycelium seemed to be loosed, and cell membrane permeability of Abasidia coerulea mycelium was improved. However, the viability of Abasidia coerulea mycelium could keep high level. Moreover, the yield of HC was improved over 1.25-fold as compared with that of the control (untreated cells), to give the yield of HC as 350 mg/l, when the Abasidia coerulea mycelium was treated with 0.5Mpa the atmosphere as the pressure media. Especially, the production of HC with atmosphere as the pressure media (0.5 MPa) could be increased by the addition of H2O2 (60 mmol/l); the relative yield of HC in moderate pressure was enriched by over 4.5% in comparison with the control. The major composition of bioconverted mixture was reduced. It was indicated that the new approach (moderate pressure) obtained in this work possessed a high potential for the industrial production of HC.

Keywords

Abasidia coerulea Hydrocortisone Permeability Viability Moderate Pressure Biotransformation

References

Mohammad AF, Mojtaba TY, Gholamreza SAZ, Steroids, 67, 869 (2002)
Porter RBR, Gallimore WA, Reese PB, Steroids, 64, 770 (1999)
Anmad S, Garg SK, Johri BN, Biotech. Adv., 10, 1 (1992)
Dordick JS, Enzyme. Microb. Technol., 11, 194 (1989)
Abramov S, Aharonowitz Y, Harnik M, Enzyme. Microb. Technol., 12, 982 (1990)
Wang J, Chen CZ, Li BA, Zhang JH, Yu YT, Enzyme Microb. Technol., 22(5), 368 (1998)
Jia SR, Li B, Park YS, J. Fermen. Biosci., 2, 191 (1996)
Coelho MAZ, Belo I, Pinheiro R, Amaral AL, Mota M, Coutinho JAP, Ferreira EC, Appl. Microbiol. Biotechnol., 66(3), 318 (2004)
Manas P, Mackey BM, Appl. Environ. Microbiol., 70, 1545 (2004)
Abe F, Kato C, Horikoshi K, Trends. Microbiol., 11, 447 (1999)
Bothun GD, Knutson BL, Berberich JA, Strobel HJ, Nokes SE, Appl. Microbiol. Biotechnol., 65(2), 149 (2004)
Bartlett DH, Kato C, Horikoshi K, Res. Microbiol., 146, 697 (1995)
Sharma A, Scott JH, Cody GD, Science, 295, 1514 (2002)
Kato M, Hayashi R, Tsuda T, Eur. J. Biochem., 269, 110 (2002)
Perrier-Cornet JM, Hayert M, Gervais P, J. Appl. Microbiol., 87, 1 (1999)
Sato S, Mukataka S, Kataoka H, J. Ferment. Technol., 59, 221 (1981)
Matsui T, Shinzato N, Ypkpta H, Process Biochem., 41, 920 (2006)
Cui JD, Li Y, Korean J. Chem. Eng., (In print) (2009)
Byun IG, Nam HU, Song SK, Hwang IS, Lee TH, Park TJ, Korean J. Chem. Eng., 22(6), 917 (2005)
Rhim JA, Yoon JH, Korean J. Chem. Eng., 22(2), 201 (2005)