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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received March 25, 2009
Accepted April 4, 2009

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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An alternative sequential extraction process for maximal utilization of bioactive components from Korean red ginseng

Guijae Yoo Sung Dong Lee¹ Man-Jin In² Woo Ik Hwang³ Kwang Seung Lee⁴ Eun Sil Lee⁴ Dong Chung Kim^5† Hee Jeong Chae^†

Department of Food and Biotechnology, Center for Food Function and Safety, Hoseo University, Asan 336-795, Korea ¹Department of Food and Nutrition, College of Health Science, Korea University, Seoul 136-703, Korea ²Department of Human Nutrition and Food Science, Chungwoon University, Hongseong 350-701, Korea ³Department of Biochemistry, College of Medicine, Korea University, Seoul 136-701, Korea ⁴NongHyup Korea Insam Co., Ltd, Jeungpyeong 122-1, Korea ⁵Institute of Basic Science, Sungkyunkwan University, Suwon 440-746, Korea

kimdc@skku.edu

Korean Journal of Chemical Engineering, July 2009, 26(4), 1094-1097(4), 10.1007/s11814-009-0182-y

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Abstract

Two types of sequential extraction process (SEP) for the recovery of bioactive components from Korean red ginseng were examined. As a SEP (normal SEP, nSEP), Korean red ginseng was sequentially treated using hot water and n-hexane for the successive extraction of water-soluble and lipid-soluble components. Also by changing the sequential order of solvents, an alternative SEP (reverse SEP, rSEP) in which n-hexane extraction was followed by hot water extraction, was proposed. Regardless of the sequential order of solvents, the recovery yield of acidic polysaccharide (AP) and crude saponin (CS) showed no significant change. On the other hand, in the rSEP, the lipid-soluble fraction was obtained from red ginseng with an enhanced recovery yield, four times higher than that in nSEP. Additionally, from dose-response assays to assess the effects of lipid-soluble components on the proliferation of human hepatoma (HepG2) and breast (MCF-7) cancer cell lines, it was found that hexane extract of red ginseng (HER) in rSEP_x000D_ has higher efficacy than the hexane extract of red ginseng marc (HERM) obtained in nSEP. This strongly suggested that rSEP would be a more attractive industrial process in terms of the efficacy of lipid-soluble extract as well as the recovery yield.

Keywords

Sequential Extraction Process Red Ginseng Normal SEP Reverse SEP

References

Shi W, Wang Y, Li J, Zhang H, Ding L, Food Chem., 102, 664 (2007)
Bahrke MS, Morgan WR, Sports Med., 29, 113 (2000)
Lee JE, Lee EJ, Kim DH, Lee JH, Yoo JH, Koh BH, J. Ethnopharmacol., 122, 143 (2009)
Park SE, Park C, Kim SH, Hossain MA, Kim MY, Chung HY, Son WS, Kim GY, Choi YH, Kim ND, J. Ethnopharmacol., 121, 304 (2009)
Oh CH, Kang PS, Vim JW, Kwon J, Oh SH, Food Sci. Biotechnol., 15, 369 (2006)
Lee JY, Hwang WI, Lim ST, J. Ethnopharmacol., 93, 409 (2004)
Choi HJ, Yee SB, Park SE, Im E, Jung JH, Chung HY, Choi YH, Kim ND, Cancer Lett., 232, 214 (2006)
Yang BW, Han ST, Ko SK, Kor. J. Pharmacogn., 37, 217 (2006)
Kim CS, Choi KJ, Yang JW, Kim SB, Korean J. Medicinal Crop Sci., 8, 146 (2000)
Sung HS, Yang CB, Kim WJ, Korean J. Food Sci. Technol., 17, 265 (1985)
Masuko T, Minami A, Iwasaki N, Majima T, Nishimura S, Lee YC, Anal. Biochem., 339, 69 (2005)
Chang EJ, Park TK, Han YN, Hwang KH, Kor. J. Pharmacogn., 38, 56 (2007)
Kwon JH, Lee GD, Belanger JMR, Pare JRJ, Inter. J. Food Sci. Technol., 38, 615 (2003)
Itano N, Atsumi F, Sawai T, Yamada Y, Miyaishi O, Senga T, Hamaguchi M, Kimata K, Proc. Natl. Acad. Sci. USA, 99, 3609 (2002)
Kim DC, Lee SD, Chae HJ, In MJ, Choi SY, Lee KS, Lee ES, Kim JH, Hwang WI, Korea Patent, 10-2009-0028100 (2009)