DNA microarray analysis of gene expression is useful for discriminating between the various subtypes of cancer, which is necessary for the accurate diagnosis and treatment of patients. Particularly, assigning biological samples into subclasses or obtaining detailed phenotypes is an important practical application for microarray gene expression profiles. In the present study, a hierarchical framework of a nonlinear mapping classification was developed for elucidating data and classifying multiclass cancers based on microarray data sets. This classification maps the gene expression profiles of multi-class cancers to the visualized latent space and predicts the clinical output through highdimensional computational biology. The proposed method was used to interpret and analyze four leukemia subtypes from microarray data. The results demonstrate that, using a high-dimensional nonlinear mapping to extract biological insights from microarray data, the proposed method can identify leukemia subtypes on the basis of molecular-level_x000D_ monitoring and improve the interpretability of leukemia clinical outputs. Furthermore, this nonlinear mapping of cancer subtypes is used to establish a relationship between expression-based subclasses of leukemia tumors and leukemia patient treatment outcomes. The proposed method may be used to guide efficient and effective approaches for the treatment of leukemia subclasses.

Bioinformatics Cancer Classification Clinical Outcome Hierarchical Framework Generative Topographic Mapping Microarray Gene Expression

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