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Received May 14, 2009
Accepted June 22, 2009
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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A bioreactor with an internal contactor for primary recovery of bovine serum albumin from yeast suspension
Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea 1Department of Chemical Engineering, Kyonggi University, Suwon 443-760, Korea
sihong@korea.ac.kr
Korean Journal of Chemical Engineering, September 2009, 26(5), 1323-1327(5), 10.1007/s11814-009-0285-5
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Abstract
In this paper the internal contactor is a newly developed device for the primary recovery of protein from crude feedstock. Ion exchanges (DEAE-Streamline) are confined inside the internal contactor in a stirred tank. Interactions between the ion exchange in the internal contactor and protein (BSA) in yeast suspension have been studied. For better performance, two strategies are considered: to determine the ion conductivity of a simulated yeast suspension, and to select the optimum process time for adsorption. In this system, advantages of both batch adsorption and expanded bed adsorption were obtained. Furthermore, in denser cell concentration (50 g/L) where EBA cannot be operated, the primary recovery was carried out in 1-2 hr. The efficiency of yield is higher than 80% in this condition.
References
Lin DQ, Fernandez-Lahore HM, Kula M, Thommes J, Bioseparation, 10, 7 (2001)
Belter PA, Cussler EL, Hu W, Bioseparations: Downstream processing for biotechnology, Wiley-Interscience (1988)
Bruce LJ, Chase HA, Chem. Eng. Sci., 56(10), 3149 (2001)
Cang S, Sanada M, Johdo O, Ohta S, Nagamatsu Y, Yoshimoto A, Biotechnol. Lett., 22(22), 1761 (2000)
Kim CH, Kim SW, Hong SI, Process Biochem., 35, 485 (1999)
Hjorth R, Expanded-bed adsorption in industrial bioprocessing: recent developments, TIBTECH, 15 (1997)
Hamilton GE, Leuchau F, Burton SC, Lyddiatt A, Journal of Biotechnology, 79, 103 (2000)
Aboudzadeh MR, Aboudzadeh N, Jiawen Z, Bin W, Korean J. Chem. Eng., 24(4), 641 (2007)
Yee L, Blanch HW, Biotechnology, 10, 109 (1992)
Bae J, Moon H, Oh KK, Kim CH, Lee DS, Kim SW, Hong SI, Biotechnol. Lett., 23(16), 1315 (2001)
Gonzalez G, Castro B, Massaldi H, Biotechnol. Bioeng., 57, 39 (1997)
Hunter AK, Carta G, Journal of Chromatography A, 930, 79 (2001)
Li P, Xiu GH, Rodrigues AE, AIChE J., 51(11), 2965 (2005)
Fernandez-Lahore HM, Geilenkirchen S, Boldt K, Nagel A, Kula MR, Thommes J, Journal of Chromatography A, 873, 195 (2000)
Belter PA, Cussler EL, Hu W, Bioseparations: Downstream processing for biotechnology, Wiley-Interscience (1988)
Bruce LJ, Chase HA, Chem. Eng. Sci., 56(10), 3149 (2001)
Cang S, Sanada M, Johdo O, Ohta S, Nagamatsu Y, Yoshimoto A, Biotechnol. Lett., 22(22), 1761 (2000)
Kim CH, Kim SW, Hong SI, Process Biochem., 35, 485 (1999)
Hjorth R, Expanded-bed adsorption in industrial bioprocessing: recent developments, TIBTECH, 15 (1997)
Hamilton GE, Leuchau F, Burton SC, Lyddiatt A, Journal of Biotechnology, 79, 103 (2000)
Aboudzadeh MR, Aboudzadeh N, Jiawen Z, Bin W, Korean J. Chem. Eng., 24(4), 641 (2007)
Yee L, Blanch HW, Biotechnology, 10, 109 (1992)
Bae J, Moon H, Oh KK, Kim CH, Lee DS, Kim SW, Hong SI, Biotechnol. Lett., 23(16), 1315 (2001)
Gonzalez G, Castro B, Massaldi H, Biotechnol. Bioeng., 57, 39 (1997)
Hunter AK, Carta G, Journal of Chromatography A, 930, 79 (2001)
Li P, Xiu GH, Rodrigues AE, AIChE J., 51(11), 2965 (2005)
Fernandez-Lahore HM, Geilenkirchen S, Boldt K, Nagel A, Kula MR, Thommes J, Journal of Chromatography A, 873, 195 (2000)
