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Received October 30, 2008
Accepted February 16, 2009
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Application of [hmim][BF4] ionic liquid in reversed-phase simulated moving bed for the separation of 3- and 4-hydroxybenzoic acid
1Department of Biological Engineering, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon, Korea 2Department of Chemical Engineering, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon, Korea 3Center for Advanced Bioseparation Technology, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon, Korea
Korean Journal of Chemical Engineering, September 2009, 26(5), 1334-1340(7), 10.1007/s11814-009-0210-y
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Abstract
In reversed-phase liquid chromatography (RP-LC), the separation of ionized compounds at neutral pH of the mobile phase is ineffective because these compounds easily become ionized and too hydrophilic to adsorb on the hydrophobic C18 stationary phase. The common method to separate ionized compounds in RP-LC is the usage of the ion pairing agent and the control of pH in the mobile phase. In this work, [hmim][BF4] ionic liquid was adopted as a mobile phase additive in reversed-phase simulated moving bed chromatography (RP-SMB) to overcome the problem mentioned above. The results showed that when 1.5 ml/l [hmim][BF4] was added to mobile phase, 3-hydroxy benzoic acid (3-HBA) and 4-hydroxy benzoic acid (4-HBA) were separated at neutral pH in the RP-SMB. In particular, purities of 3-HBA and 4-HBA products were 97.62% and 100%, respectively, and had a good agreement with simulation results.
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References
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Lough WJ, Wainer IW, High performance liquid chromatography fundamental principles and practice, Blackie Academic and Professional, London (1995)
