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Received December 17, 2008
Accepted March 29, 2009
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Rapid differentiation of new isolates with MALDI-TOF mass spectrometry via discriminant function analysis based on principal components
Department of Bioengineering, Marmara University, Kadikoy 34722, Istanbul, Turkey
Korean Journal of Chemical Engineering, November 2009, 26(6), 1645-1651(7), 10.1007/s11814-009-0243-2
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Abstract
Discriminant function analysis based on principal components was applied to the spectral outputs of whole cell suspensions of nine isolates from matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. First, based on the salt tolerance and whole cell proteins, the similarity of the isolates to moderate halophiles was established. Intact microorganisms were then inferentially clustered by MALDI-TOF mass spectroscopy taking_x000D_
four type strains as precursors. Two of these type strains were moderate halophilic bacteria (Halomonas salina and Halomonas halophila), one was a mesophilic bacteria (Escherichia coli), and one was a halophilic archaea (Haloarcula vallismortis). Results showed that the isolates were significantly similar to halophiles but were different from a mesophile. This investigation demonstrates the feasibility of using whole cell suspensions for rapid differentiation prior_x000D_
to extensive experimentation.
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References
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Kell DB, Brown M, Davey HM, Dunn WB, Spasic I, Oliver SG, Nat. Rev. Microbiol., 3, 557 (2005)
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Allen J, Davey HM, Broashurst D, Heald JK, Rowland JJ, Oliver SG, Nat. Biotechnol., 21, 692 (2003)
Timmins EM, Howell AS, Alsberg BK, Noble WC, Goodacre R, J. Clin. Microbiol., 36, 367 (1998)
Vaidyanathan S, Rowland JJ, Kell DB, Goodacre R, Anal. Chem., 73, 4134 (2001)
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Laemmli UK, Nature, 227, 680 (1970)
Blum H, Beier H, Gross HJ, Electrophoresis, 8, 93 (1987)
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Ventosa A, Nieto JJ, Oren A, Microbiol. Mol. Biol. R., 62, 504 (1998)
Fukuchi S, Yoshimune K, Wakayama M, Moriguchi M, Nishikawa K, J. Mol. Biol., 327, 347 (2003)
Yeung KY, Ruzzo WL, Bioinformatics, 17, 763 (2001)
