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Received March 30, 2009
Accepted May 7, 2009
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Simple preparation of immobilized-metal affinity chromatography media
Department of Chemical Engineering, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Korea
inkim@cnu.ac.kr
Korean Journal of Chemical Engineering, November 2009, 26(6), 1693-1695(3), 10.1007/s11814-009-0276-6
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Abstract
Immobilized-metal affinity chromatography (IMAC) media was prepared. Iminodiacetic acid (IDA) was optimally coupled to the oxirane-activated gel at pH 13.0 and 60 ℃ in 0.1-0.15 g of IDA per ml of 2M Na2CO3 for 5-7 hours. The amount of coupled IDA was 600-800 micromoles per gram of dried gel by determining zinc (II) ion with atomic absorption spectroscopy. Adsorption and desorption of protein sample to IDA-coupled media was made and the result is compatible to ones reported previously. The efficiency of column chromatography was discussed on partially purifying β-galactosidase from E. coli as the protein sample by zinc (II) ion chelate affinity column.
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