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In relation to this article, we declare that there is no conflict of interest.
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Received April 30, 2009
Accepted June 1, 2009
articles This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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A simple and cost-effective method to prepare template DNAs for cell-free protein synthesis

Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea 1Department of Chemical System Engineering, Hongik University, Jochiwon, Chungnam 339-701, Korea
dmkim@cnu.ac.kr
Korean Journal of Chemical Engineering, January 2010, 27(1), 179-182(4), 10.1007/s11814-009-0294-4
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Abstract

The scale-up of cell-free protein synthesis reactions involves the preparation of large amounts of template DNA. While ion-exchange column chromatography methods have commonly been used to obtain purified plasmid DNA for cell-free protein synthesis reactions, these methods are costly and difficult to expand to a large scale. In this work, we report that the routine isopropyl alcohol (IPA) precipitation method can be used to prepare cell-free-expressible DNA when the co-precipitated proteins are removed. Compared to column-purification procedures, the IPA-precipitation offers obvious advantages with respect to the cost and scaling-up of template preparation, and we believe that our finding will contribute to making cell-free protein synthesis system more practical for the rapid production of preparative amounts of recombinant proteins.

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