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Received April 30, 2009
Accepted July 1, 2009
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Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway
College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-OrientedChemical Engineering, Nanjing University of Technology, Nanjing 210009, P. R. China
biotech@njut.edu.cn
Korean Journal of Chemical Engineering, January 2010, 27(1), 183-186(4), 10.1007/s11814-009-0323-3
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Abstract
Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21.1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased_x000D_
by 83.7%.
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Tsao GT, Cao NJ, Du J, Gong CS, Adv. Biochem. Eng. Biot., 65, 243 (1999)
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Gangl IC, Weigand WA, Keller FA, Appl. Biochem. Biotech., 28, 471 (1991)
Bai DM, Zhao XM, Li XG, Xu SM, Biochem. Eng. J., 18, 41 (2004)
