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Received May 19, 2009
Accepted June 10, 2009
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Bio-hydrogen production from a marine brown algae and its bacterial diversity
Department of Pharmaceutical Engineering, College of Medical Bioscience, Silla University, Busan 617-736, Korea
jhalee@silla.ac.kr
Korean Journal of Chemical Engineering, January 2010, 27(1), 187-192(6), 10.1007/s11814-009-0300-x
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Abstract
The aim of this study was to determine how bio-hydrogen production was related to the composition of the bacterial community in a dark fermentation fed with marine brown algae (Laminaria japonica). The bacterial diversity was ascertained by 16S rDNA PCR-sequencing. A total of 444 mL of bio-hydrogen was produced from 10 g/L of dry algae in a 100 mL of culture fluid for 62 h. The pH varied from 8.74 to 7.05. Active bio-hydrogen production was observed from 24 to 48 h, and maximum bio-hydrogen production was 106 mL over 1 L gas. The bacterial community_x000D_
of the activated sludge consisted of 6 phyla, where H2 producing and consuming bacteria coexisted. The only detectable bacterial phylum after bio-hydrogen generation with heat-treated (65 ℃, 20 min) seeding was Firmicutes. Clostridium and Bacillus species constituted 54% and 46%, respectively, of the bacterial mixture and the most abundant species was Clostridium beijierinckii (34%). These results may provide a better understanding of how different biohydrogen_x000D_
communities affect hydrogen production and aid in the optimization of bio-hydrogen production.
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Klass DL, Chem. Tech., 3, 161 (1974)
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Wagner M, Amann R, Lemmer H, Schleifer KH, Appl. Environ. Microbiol., 59, 1520 (1993)
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Fang HHP, Liu H, Bioresour. Technol., 82(1), 87 (2002)
Ueno Y, Otsuka S, Morimoto M, J. Ferment. Bioeng., 82(2), 194 (1996)
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Singh A, Pandey KD, Dubey RS, Int. J. Hydrogen Energy, 24, 693 (1999)
McTavish H, J. Biochem., 123, 644 (1998)
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Oh YK, Seol EH, Lee EY, Park S, Int. J. Hydrogen Energy, 27, 1373 (2002)
Nandi R, Sengupta S, Crit. Rev. Microbiol., 24, 61 (1998)
