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Received June 2, 2009
Accepted July 29, 2009
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Effects of nitrogen source and carbon/nitrogen ratio on batch fermentation of glutathione by Candida utilis
School of Life Science, Soochow University, Suzhou 215123, P. R. China 1Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, P. R. China
Korean Journal of Chemical Engineering, February 2010, 27(2), 551-559(9), 10.1007/s11814-010-0065-2
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Abstract
Several inorganic/organic N-containing substances were tested as nitrogen source for efficient glutathione production by C. utilis WSH 02-08. Although the strain could assimilate all the inorganic/organic nitrogen, urea and ammonium sulfate were found more favorable to cell growth and glutathione biosynthesis in a flask, respectively, and an optimal C/N ratio existed for each as 5.6 mol/mol and 8.3 mol/mol. A mixed nitrogen source of urea and ammonium under diverse C/N ratios could not boost glutathione fermentation despite the many mixed strategies that were introduced. Batch glutathione production in a stirred fermentor, using the sole or mixed nitrogen sources of urea and ammonium sulfate under their optimal C/N ratios, were conducted; urea was further proved to be the best nitrogen source for glutathione production. The reason was then quantitatively described by kinetic model, together with the distribution of flux for metabolites in metabolic network of glutathione biosynthesis by C. utilis WSH 02-08.
References
Meister A, Anderson ME, Annu. Rev. Biochem., 52, 711 (1983)
Yamauchi A, Tsuyuki S, Inamoto T, Yamaoka Y, Antioxid. Redox Sign., 1, 245 (1999)
Pastore A, Federici G, Bertini E, Piemonte F, Clin. Chim. Acta, 333, 19 (2003)
Sies H, Free Rad. Bio. Med., 27, 916 (1999)
Lafleur MV, Hoorweg JJ, Joenje H, Westmijze EJ, Retel J, Free Radical Res., 21, 9 (1994)
Sen CK, J. Nutr. Biochem., 8, 660 (1997)
Villarama CD, Maibach HI, Int. J. Cosmetic Sci., 27, 147 (2005)
Li Y, Wei GY, Chen J, Appl. Microbiol. Biotechnol., 66(3), 233 (2004)
van Urk H, Voll WSL, Sheffers WA, van Dijken JP, Appl. Environ. Microbiol., 56, 281 (1990)
Castrillo JI, Kaliterna J, Weusthuis RA, Vandijken JP, Pronk JT, Biotechnol. Bioeng., 49(6), 621 (1996)
Jeffries TW, Jin YS, Appl. Microbiol. Biotechnol., 63(5), 495 (2004)
Wen SH, Zhang T, Tan TW, Enzyme Microb. Technol., 35(6-7), 501 (2004)
Wei GY, Wang DH, Chen J, Biotechnol. Bioprocess Eng., 13, 347 (2008)
KHAN JA, ABULNAJA KO, KUMOSANI TA, ABOUZAID AZA, Bioresour. Technol., 53(1), 63 (1995)
Devine ST, Slaughter TC, FEMS Microbiol. Lett., 9, 19 (1980)
Tietze F, Anal. Biochem., 27, 502 (1969)
Li Y, Chen J, Liang DF, Lun SY, J. Biotechnol., 81, 27 (2000)
Wei GY, Li Y, Du GC, Chen J, J. Chem. Ind. Eng. (China), 57, 1410 (2006)
Nielsen J, Biotechnol. Bioeng., 58(2-3), 125 (1998)
Lee ES, Park JY, Yeom SH, Yoo YJ, Korean J. Chem. Eng., 25(1), 139 (2008)
Thomas KC, Hynes SH, Ingledew WM, Biotechnol. Lett., 18(10), 1165 (1996)
Wei GY, Wang DH, Chen J, J. Chem. Ind. Eng. (China), 58, 2329 (2007)
Jr. Gaden EL, J. Biochem. Microbiol. Technol. Eng., 1, 413 (1959)
Yamauchi A, Tsuyuki S, Inamoto T, Yamaoka Y, Antioxid. Redox Sign., 1, 245 (1999)
Pastore A, Federici G, Bertini E, Piemonte F, Clin. Chim. Acta, 333, 19 (2003)
Sies H, Free Rad. Bio. Med., 27, 916 (1999)
Lafleur MV, Hoorweg JJ, Joenje H, Westmijze EJ, Retel J, Free Radical Res., 21, 9 (1994)
Sen CK, J. Nutr. Biochem., 8, 660 (1997)
Villarama CD, Maibach HI, Int. J. Cosmetic Sci., 27, 147 (2005)
Li Y, Wei GY, Chen J, Appl. Microbiol. Biotechnol., 66(3), 233 (2004)
van Urk H, Voll WSL, Sheffers WA, van Dijken JP, Appl. Environ. Microbiol., 56, 281 (1990)
Castrillo JI, Kaliterna J, Weusthuis RA, Vandijken JP, Pronk JT, Biotechnol. Bioeng., 49(6), 621 (1996)
Jeffries TW, Jin YS, Appl. Microbiol. Biotechnol., 63(5), 495 (2004)
Wen SH, Zhang T, Tan TW, Enzyme Microb. Technol., 35(6-7), 501 (2004)
Wei GY, Wang DH, Chen J, Biotechnol. Bioprocess Eng., 13, 347 (2008)
KHAN JA, ABULNAJA KO, KUMOSANI TA, ABOUZAID AZA, Bioresour. Technol., 53(1), 63 (1995)
Devine ST, Slaughter TC, FEMS Microbiol. Lett., 9, 19 (1980)
Tietze F, Anal. Biochem., 27, 502 (1969)
Li Y, Chen J, Liang DF, Lun SY, J. Biotechnol., 81, 27 (2000)
Wei GY, Li Y, Du GC, Chen J, J. Chem. Ind. Eng. (China), 57, 1410 (2006)
Nielsen J, Biotechnol. Bioeng., 58(2-3), 125 (1998)
Lee ES, Park JY, Yeom SH, Yoo YJ, Korean J. Chem. Eng., 25(1), 139 (2008)
Thomas KC, Hynes SH, Ingledew WM, Biotechnol. Lett., 18(10), 1165 (1996)
Wei GY, Wang DH, Chen J, J. Chem. Ind. Eng. (China), 58, 2329 (2007)
Jr. Gaden EL, J. Biochem. Microbiol. Technol. Eng., 1, 413 (1959)
