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Received March 23, 2009
Accepted May 24, 2009
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Transfer rate measurement of lysozyme by liquid-liquid extraction using reverse micelles with dense CO2
Sun-Mi Jung
Un-Mi Shin
Md. Salim Uddin
Sun-Young Park1
Hideki Kishimura2
Gordon Wilkinson3
Byung-Soo Chun†
Institute of Food Science, Faculty of Food Science & Biotechnology, Pukyong National University, Busan 608-737, Korea 1School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5001, Australia 2Research Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan 3School of Chemical Engineering, University of Adelaide, Adelaide, SA 5005, Australia
bschun@pknu.ac.kr
Korean Journal of Chemical Engineering, February 2010, 27(2), 596-601(6), 10.1007/s11814-010-0063-4
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Abstract
Lysozyme was extracted from aqueous solution into i-octane using reverse micelles in the presence of pressurized CO2. A squat vessel with two independent stirrers was used to measure the mass transfer of the lysozyme across a planar interface. Mass transfer coefficient, k(L) of the lysozyme from the aqueous to the organic phase was measured at selected ionic strengths, pH, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) surfactant concentrations, temperatures and pressurized CO2. The mass transfer rate of lysozyme was higher in high temperature (318 K) and pressure (20MPa). pH of 9 in aqueous phase showed highest mass transfer rate of lysozyme. The application of pressurized CO2 markedly increased the mass transfer rate of lysozyme comparing to conventional non-pressurized system.
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References
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Asai S, Hatanaka J, Uekawa Y, J. Chem. Eng. Jpn., 16, 463 (1963)
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Schneider GM, in Supercritical Fluids Fundamentals for Application, Kiran E, Sengers JMHL, Eds., Kluwer Academic Publishers, Boston, USA (1994)
