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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received May 11, 2009
Accepted July 3, 2009

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Removal of neurotoxic ethyl parathion pesticide by two-stage chemical/enzymatic treatment system using Fenton’s reagent and organophosphorous hydrolase

Suk Soon Choi^† Sang Hwan Seo Dong Gyun Kang¹ Jeong Hyub Ha² Hyung Joon Cha^1†

Department of Biological and Environmental Engineering, Semyung University, Jecheon 390-711, Korea ¹National Research Laboratory of Molecular Biotechnology, Department of Chemical Engineering, Pohang University of Science and Technology, Pohnag 790-784, Korea ²Department of Environmental Science, Kangwon National University, Chuncheon 200-701, Korea

Korean Journal of Chemical Engineering, March 2010, 27(3), 900-904(5), 10.1007/s11814-010-0115-9

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Abstract

Organophosphate compounds, which are essential ingredients of pesticide, plasticizer, air fuel, and chemical warfare agents, are serious neurotoxic hazardous materials. Therefore, diverse physical-chemical treatments have been attempted to degrade organophosphate compounds. In the present work, we propose a two-stage chemical and enzymatic treatment system. As a first stage, pretreatment of oxidation and coagulation using Fenton’s reagent utilizing iron and hydrogen peroxide was employed. Preferentially, 1 mM ethyl parathion (EP) pesticide was largely (~80%) removed by Fenton’s reagent reaction within 15 min. To remove residual EP, enzymatic treatment with organophosphorous hydrolase (OPH) from recombinant Escherichia coli was employed as a second stage. We successfully demonstrated that the proposed two-stage hybrid treatment process removed 1mM environmental toxic EP efficiently (~98%) within 30 min.

Keywords

Organophosphate Compound Ethyl Parathion Pesticide Two-stage Treatment Fenton’s Reagent Organophosphorous Hydrolase

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