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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received July 1, 2009
Accepted October 25, 2009

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Regulation of meilingmycin in Streptomyces nanchangensis: Effect of ammonium ion

Yong Wang Ying-ping Zhuang^† Ping Wang Ju Chu Si-liang Zhang

State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology, East China University of Science and Technology, Shanghai 200237, P. R. China

Korean Journal of Chemical Engineering, March 2010, 27(3), 910-914(5), 10.1007/s11814-010-0150-6

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Abstract

Effects of different ammonium sulfate concentrations on meilingmycin biosynthesis were studied in this research. The results show that a lower concentration of ammonium ions stimulates the biosynthesis of meilingmycin, while a concentration higher than 5mmol/L inhibits the mycelial growth and the biosynthesis of the products. However, increased sugar consumption rate with the elevated concentration of ammonium sulfate was observed during the fermentation process. On this basis, six enzymes, which are responsible for the meilingmycin biosynthesis and the glucose metabolism, were measured and analyzed during the bioprocess. The results suggest that glucose-6-phosphate dehydrogenase, citrate synthase, succinate dehydrogenase and fatty acid synthase are stimulated by higher concentration of ammonium ions, while valine dehydrogenase and methylmalonyl-CoA carboxyltransferase are inhibited. From the results it follows that the precursor supply was restricted with the higher concentration of ammonium ions, which results in the lower production of meilingmycin. Thus, the strategy of maintaining a low level of FAS activity is a critical factor for high yield of meilingmycin.

Keywords

Meilingmycin Streptomyces nanchangensis Ammonium Ion

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