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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received July 23, 2009
Accepted October 20, 2009

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Melanogenesis inhibitory effect of dehydroevodiamine isolated from fruits of Evodia rutaecarpa

Lian Hua Luo^{1 2} Jeong-Woo Seo¹ Dung Hoang Nguyen³ Eun-Ki Kim³ Soon Ah Kang⁴ Dae-Hyuk Kim² Chul Ho Kim^1†

¹Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongup, Korea ²Institute for Molecular Biology and Genetics, Research Center of Bioactive Materials, Chonbuk National University, Jeonju, Korea ³Department of Biological Engineering, National Lab of Skin Bioactive Material, Inha University, Incheon, Korea ⁴Department of Fermented Food Science, Seoul University of Venture and Information, Seoul, Korea

kim3641@kribb.re.kr

Korean Journal of Chemical Engineering, March 2010, 27(3), 915-918(4), 10.1007/s11814-010-0147-1

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Abstract

Dehydroevodiamine, an alkaloid, was isolated from the fruit of Evodia rutaecarpa and melanin production, and tyrosinase inhibition in B16F10 melanoma cells treated with the isolated dehydroevodiamine was investigated. The compound decreased melanin synthesis significantly without promoting cytotoxicity. The IC50 value of dehydroevodiamine for melanogenesis and cell viability were 59.8 μM and 90.0 μM, respectively. The L-dopa oxidase activity of mushroom tyrosinase was reduced after dehydroevodiamine treatment by about 22.4% at a concentration of 33.2 μM. However, there was no effect on cellular tyrosinase activity. These results indicate that the observed decrease in melanin content after treatment with dehydroevodiamine was attributed to the direct inhibition of tyrosinase activity, rather than the suppression of tyrosinase gene expression. Dehydroevodiamine may be a promising new agent for use in cosmeceutical application.

Keywords

Melanogenesis Evodia rutaecarpa Dehydroevodiamine Skin Whitening Cosmeceutical

References

Tian WX, Dong YQ, Hu C, Wen F, Physiol. Rev., 84, 1155 (2004)
Ito S, Wakamatsu K, Photochem. Photobiol., 84, 582 (2008)
Schallreuter KU, Kothari S, Chavan B, Spencer JD, Exp. Dermatol., 17, 395 (2008)
Yamaguchi Y, Takahashi K, Zmudzka BZ, Kornhauser A, Miller SA, Tadokoro T, Berens W, Beer JZ, Hearing VJ, Faseb J., 20, 1486 (2006)
Parvez S, Kang M, Chung HS, Bae H, Phytother. Res., 21, 805 (2007)
Yang XW, Teng J, J. Chin. Pharmaceut. Sci., 16, 20 (2007)
Yang XW, Teng J, Wang Y, Xu W, Phytother. Res., 23, 56 (2009)
Zhang H, Yang XW, Cui YX, Chin. J. Magn. Reson., 16, 563 (1999)
Zhang QH, Gao HY, Wu LJ, Zhang L, J. Shen. Pharm. Univ., 22, 12 (2005)
Kai H, Baba M, Okuyama T, Planta. Med., 74, 1785 (2008)
Arung ET, Shimizu K, Kondo R, Biol. Pharm. Bull., 29, 1966 (2006)
Zhang X, Hu X, Hou A, Wang H, Biol. Pharm. Bull., 32, 86 (2009)
Nguyen DH, Nguyen DTM, La LH, Yang SH, Lee HB, Kim HJ, Shin JH, Kim DM, Kim EK, Korean J. Chem. Eng., 24(5), 827 (2007)
Sato K, Takahashi H, Iraha R, Toriyama M, Biol. Pharm. Bull., 31, 33 (2008)
Kong YH, Jo YO, Cho CW, Son D, Park S, Rho J, Choi SY, Biol. Pharm. Bull., 31, 946 (2008)
Cho Y, Kim KH, Shim JS, Hwang JK, Biol. Pharm. Bull., 31, 986 (2008)
Wang HH, Chou CJ, Liao JF, Chen CF, Eur. Pharmacol., 413, 221 (2001)
Peng JH, Zhang CE, Wei W, Hong XP, Pan XP, Wang JZ, Neuropharmacology, 52, 1521 (2007)