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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received September 29, 2009
Accepted November 23, 2009

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A novel strategy on the high-cell-density cultivation of Candida utilis for the enhanced production of glutathione

Min Nie Gongyuan Wei^† Na Shao Xiaoguang Ge

School of Basic Medicine and Biological Science, College of Medicine, Soochow University, Suzhou 215123, P. R. China

Korean Journal of Chemical Engineering, July 2010, 27(4), 1246-1251(6), 10.1007/s11814-010-0190-y

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Abstract

Efficient glutathione production by high-cell-density cultivation of Candida utilis was investigated. A series of batch glutathione fermentations were carried out and the optimal initial glucose concentration was found to be about 26 g/L. Then, fed-batch fermentation under diverse feeding strategies was used to enhance glutathione production with a total glucose concentration of 150 g/L. Constant glucose feeding strategy cannot meet the requirement of cells at the late period of feeding, while exponential glucose feeding strategy cannot satisfy the needs of cells at the beginning of feeding. Based on the results above, a polynomial glucose feeding strategy was developed to provide enough glucose for cells along with the cultivation, under which both the cell and glutathione productivity were satisfactorily improved. Furthermore, fed-batch fermentation under this strategy with a total glucose concentration of 200 g/L was successfully performed, the dry cell weight and glutathione concentration reached 91.2 g/L and 825 mg/L, respectively.

Keywords

Glutathione Candida utilis Fed-batch Fermentation High-cell-density Cultivation

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