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Received January 27, 2010
Accepted March 4, 2010
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Optimization of medium components for D-ribose production by transketolase-deficient Bacillus subtilis NJT-1507
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology andPharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, P. R. China
Korean Journal of Chemical Engineering, November 2010, 27(6), 1725-1729(5), 10.1007/s11814-010-0288-2
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Abstract
Statistical experimental designs were used to optimize the composition of culture media for the production of D-ribose by Bacillus subtilis. A fractional factorial design 2(5-2) was used to determine medium components that significantly affected D-ribose production. The concentrations of glucose and (NH4)2SO4 were the significant factors. Central composite design and response surface methodology were then used to estimate the quadratic response surface_x000D_
and determine the factor levels for maximum production of D-ribose. Finally, the optimal medium composition was obtained (g/L): glucose, 172.75; (NH4) 2SO4, 13.2; yeast powder, 4; corn steep liquor, 8 and MnSO4, 0.5. This optimization strategy increased D-ribose production from 73.21 g/L to 88.57 g/L, an increase of 22% compared with the original conditions. The D-ribose production yield to glucose concentration was also enhanced from 0.37 g/g to 0.52 g/g. Confirmatory_x000D_
experiments were also performed to demonstrate the accuracy of the model. Under the optimal medium using ammonia to control pH in a 5 L fermenter, the D-ribose yield was increased to 95.28 g/L after 3 days of cultivation at 37 ℃.
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