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Received December 6, 2009
Accepted March 28, 2010
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High cell density fermentation of Saccharomyces cerevisiae GS2 for selenium-enriched yeast production
Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P. R. China
twtanbuct@sina.com
Korean Journal of Chemical Engineering, November 2010, 27(6), 1836-1840(5), 10.1007/s11814-010-0300-x
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Abstract
This paper describes a fed-batch fermentation protocol about production of selenium-enriched yeast. Saccharomyces cerevisiae GS2 was selected because of its high tolerance to selenium. The strain GS2 was tested and 122± 0.5 g·l-1 dry cell weight was obtained after 30 h cultivation through feed back control of feed rate of glucose according to the concentration of ethanol and dissolved oxygen. Furthermore, the optimal pattern of Na2SeO3 addition was 9 mg Na2SeO3 against 1 g DCW at late exponential phase. With the combination of glucose feeding and Na2SeO3 addition, the final dry cell biomass reached 102±0.4 g·l-1 and a Se uptake level of 2,020±13 mg·Kg-1 was achieved in a 5 l fermentor after 38 h cultivation.
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