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Received January 5, 2010
Accepted April 10, 2010
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Kinetic model of asymmetric reduction of 3-oxo-3-phenylpropionic acid ethyl ester using Saccharomyces cerevisiae CGMCC No.2266
Pharmaceuticals College, Zhejiang University of Technology, Hang Zhou, 310014, China
Korean Journal of Chemical Engineering, November 2010, 27(6), 1841-1846(6), 10.1007/s11814-010-0307-3
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Abstract
The kinetic model of asymmetric reduction of 3-oxo-3-phenylpropionic acid ethyl ester using Saccharomyces cerevisiae CGMCC No.2266 with 10% glucose as co-substrate to realize the regeneration of NADPH was established. The effect factors on reduction, the type and the content of co-substrate and coenzyme, and the changes of the substrate and product content vs. time during the reaction process were investigated. The results indicate that 10% glucose can increase the reaction conversion from 23.0% to 98.4% and NADPH is reducer. The reduction process conforms with sequence mechanisms. The model parameters are as follows: v(m)=5.0×10^(-4) mol·L^(-1)·h^(-1), k(1)=1.5×10^(-6) mol·L^(-1)·h^(-1), k(2)=3.0×10^(-3) mol·L^(-1)·h^(-1). The kinetic model is in good agreement with the experimental data.
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References
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