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Received February 8, 2010
Accepted April 4, 2010
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Screening of Candida utilis and medium optimization for co-production of S-adenosylmethionine and glutathione
School of Basic Medicine and Biological Science, Soochow University, Suzhou Industrial Park, Suzhou, 215123, Jiangsu Province, P. R. China
Korean Journal of Chemical Engineering, November 2010, 27(6), 1847-1853(7), 10.1007/s11814-010-0305-5
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Abstract
An effective S-adenosylmethionine and glutathione enriching yeast mutant of Candida utilis CCTCC M 209298 was first screened from plates containing 0.5 g/L of DL-ethionine by complex mutagenesis with UV and γ-ray in this study. Medium components optimization for enhanced co-production of S-adenosylmethionine and glutathione by C. utilis CCTCC M 209298 was further carried out using response surface methodology. The significant factors influencing S-adenosylmethionine and glutathione co-production were selected by Plackett-Burman design as_x000D_
sucrose, KH2PO4 and L-methionine, and Box-Behnken design was applied for further optimization studies. Based on these approaches, the optimized concentrations on medium components for higher co-production of S-adenosylmethionine and glutathione were sucrose 35.4 g/L, (NH4)2SO4 10 g/L, KH2PO4 12.3 g/L, MgSO4·7H2O, 0.05 g/L, CaCl2 0.05 g/L and L-methionine 4.6 g/L. The medium optimization by response surface methodology led to a total production of 589.3 mg/L on S-adenosylmethionine and glutathione, which was 2.4-fold increased compared with the medium without optimization.
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Yoon GS, Ko KH, Kang HW, Suh JW, Kim YS, Ryu YW, Enzyme Microb. Technol., 39(3), 466 (2006)
