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Received February 17, 2010
Accepted May 11, 2010
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Silk fibroin microspheres prepared by the water-in-oil emulsion solvent diffusion method for protein delivery
Department of Chemistry and Center of Excellence for Innovation in Chemistry,Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand
Korean Journal of Chemical Engineering, January 2011, 28(1), 293-297(5), 10.1007/s11814-010-0322-4
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Abstract
Silk fibroin (SF) microspheres were prepared by the simple water-in-oil emulsion solvent diffusion method without any surfactants. Aqueous SF solution and dichloromethane were used as water and oil phases, respectively. Influence of water : oil phase ratios on SF microsphere characteristics was investigated. From FTIR spectra, the resulting SF microspheres showed predominantly random coil SF conformation. SF microspheres observed from SEM images were spherical with deflated surfaces in some cases. Particle sizes of the SF microspheres were in the range 45-92 μm. Finally, bovine serum albumin (BSA) was used as a model protein for entrapment within the SF microspheres. The BSA-loaded SF microspheres were larger than unloaded SF microspheres. In vitro release tests indicate that BSA release from the SF microspheres was influenced by BSA content.
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References
Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL, Biomaterials., 24, 401 (2003)
Tamada Y, Biomacromolecules, 6(6), 3100 (2005)
Acharya C, Ghosh SK, Kundu SC, Acta Biomater., 5, 429 (2009)
Sugihara A, Sugiura K, Morita H, Ninagawa T, Tubouchi K, Tobe R, Izumiya M, Horio T, Abraham NG, Ikehara S, Proc.Soc. Exp. Biol. Med., 225, 58 (2000)
Yoshimizu H, Asakura T, J. Appl. Polym. Sci., 40, 127 (1990)
Liu YC, Qian JH, Liu HY, Zhang XL, Deng JQ, Yu TY, J. Appl. Polym. Sci., 61(4), 641 (1996)
Hofmann S, Wong Po Foo CT, Rossetti F, Textor M, Vunjak-Novakovic G, Kaplan DL, Merkle HP, Meinel L, J. Controlled Release., 111, 219 (2006)
Yeo JH, Lee KG, Lee YW, Kim SY, Eur. Polym. J., 39, 1195 (2003)
Hino T, Tanimoto M, Shimabayashi S, J. Colloid Interface Sci., 266(1), 68 (2003)
Wang X, Wenk E, Matsumoto A, Meinel L, Li C, Kaplan DL, J. Controlled Release., 117, 360 (2007)
Zhang YQ, Biotechnol. Adv., 16, 961 (1998)
Zhang YQ, Zhu J, Gu RA, Appl. Biochem. Biotechnol., 75(2-3), 215 (1998)
Lv Q, Cao CB, Zhang Y, Ma XL, Zhu HS, J. Appl. Polym. Sci., 96(6), 2168 (2005)
Zuo BQ, Liu L, Wu Z, J. Appl. Polym. Sci., 106(1), 53 (2007)
Li XW, Yang TF, Korean J. Chem. Eng., 25(5), 1201 (2008)
Zulegar S, Lippold BC, Int. J. Pharm., 217, 139 (2001)
Tamada Y, Biomacromolecules, 6(6), 3100 (2005)
Acharya C, Ghosh SK, Kundu SC, Acta Biomater., 5, 429 (2009)
Sugihara A, Sugiura K, Morita H, Ninagawa T, Tubouchi K, Tobe R, Izumiya M, Horio T, Abraham NG, Ikehara S, Proc.Soc. Exp. Biol. Med., 225, 58 (2000)
Yoshimizu H, Asakura T, J. Appl. Polym. Sci., 40, 127 (1990)
Liu YC, Qian JH, Liu HY, Zhang XL, Deng JQ, Yu TY, J. Appl. Polym. Sci., 61(4), 641 (1996)
Hofmann S, Wong Po Foo CT, Rossetti F, Textor M, Vunjak-Novakovic G, Kaplan DL, Merkle HP, Meinel L, J. Controlled Release., 111, 219 (2006)
Yeo JH, Lee KG, Lee YW, Kim SY, Eur. Polym. J., 39, 1195 (2003)
Hino T, Tanimoto M, Shimabayashi S, J. Colloid Interface Sci., 266(1), 68 (2003)
Wang X, Wenk E, Matsumoto A, Meinel L, Li C, Kaplan DL, J. Controlled Release., 117, 360 (2007)
Zhang YQ, Biotechnol. Adv., 16, 961 (1998)
Zhang YQ, Zhu J, Gu RA, Appl. Biochem. Biotechnol., 75(2-3), 215 (1998)
Lv Q, Cao CB, Zhang Y, Ma XL, Zhu HS, J. Appl. Polym. Sci., 96(6), 2168 (2005)
Zuo BQ, Liu L, Wu Z, J. Appl. Polym. Sci., 106(1), 53 (2007)
Li XW, Yang TF, Korean J. Chem. Eng., 25(5), 1201 (2008)
Zulegar S, Lippold BC, Int. J. Pharm., 217, 139 (2001)
