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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received January 24, 2011
Accepted March 29, 2011

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of operating parameters on precipitation for recovery of lactic acid from calcium lactate fermentation broth

Da-Jeong Min Kook Hwa Choi Yong Keun Chang¹ Jin-Hyun Kim^†

Department of Chemical Engineering, Kongju National University, Cheonan, Chungnam 331-717, Korea ¹Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea

Korean Journal of Chemical Engineering, October 2011, 28(10), 1969-1974(6), 10.1007/s11814-011-0082-9

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Abstract

Precipitation is a simple, efficient method for separating and recovering lactic acid in the form of calcium lactate from fermentation broth by adding sulfuric acid. Major operating parameters of the recovery step as well as the temperature of concentration of the recovered lactic acid solution and the type and amount of adsorbent used for pigment (color) removal were optimized. When the molar ratio of calcium lactate to sulfuric acid was 1 : 1 and the pH was increased to a value greater than the pKa (3.86), calcium sulfate was precipitated and could be removed more effectively, allowing for more efficient separation and recovery of supernatant lactic acid. Precipitation could be facilitated by adding calcium lactate solution with mixing (up to 220 rpm) and was completed in over 18 h. The optimal temperature for the concentration of lactic acid recovered from the supernatant after removing the precipitated calcium sulfate was found to be 90 oC in terms of the time required for concentration and the stability of the product. Activated carbon (SX-PLUS, 9 g/L) was most effective as an adsorbent for color removal from the recovered lactic acid. Under the optimized precipitation conditions, an overall yield of 92% of lactic acid from fermentation broth could be achieved.

Keywords

Lactic Acid Recovery Precipitation Operation Parameters Fermentation Broth

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