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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received June 22, 2011
Accepted August 4, 2011

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Simulation study of biobutanol production in a polymer-loaded two-phase partitioning bioreactor (PL-TPPB): Simulation and strategy for biobutanol production

Sung Ho Yeom^†

Department of Biochemical Engineering, Gangneung-Wonju National University, Gangneung, Gangwon 210-702, Korea

Korean Journal of Chemical Engineering, October 2011, 28(10), 2017-2023(7), 10.1007/s11814-011-0204-4

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Abstract

A simulation study was performed for a two-phase partitioning bioreactor (TPPB) with polymer beads, Dowex Optipore L-493, as a second phase. When the initial glucose concentration is less than 30 g/L, a single-phase bioreactor is preferred, because it consumed all the glucose with 40% of biobutanol yield. Any glucose over the concentration remained in the single-phase bioreactor because cells were completely inhibited by products, mainly biobutanol, and thus glucose availability became less than 100%. The TPPB with 10% polymer beads completely consumed up to 120 g/L glucose and more polymer beads were required for the higher glucose concentration. Instead of increasing the proportion of polymer beads, 2 vvm of nitrogen gas was introduced continuously into the TPPB for the stripping of products, reducing product inhibitions. By applying gas stripping to the TPPB containing 10% polymer beads, 150 g/L of glucose was completely consumed and 99.7% acetone, 46.8% butanol and 82.5% ethanol was stripped out of the TPPB. Finally, on the basis of these estimations, a novel strategy based on the initial glucose concentration was suggested for high biobutanol production.

Keywords

Biobutanol Product Inhibition Two-phase Partitioning Bioreactor Second Phase Polymer Beads Gas Stripping

References

Sang BI, Kim YH, News Inf. Chem. Eng., 26(6), 704 (2008)
Cha WO, News Inf. Chem. Eng., 25(6), 609 (2007)
Izak P, Ruth W, Fei Z, Dyson PJ, Kragl U, Chem. Eng. J., 139(2), 318 (2008)
Sang BI, Kim YH, News Inf. Chem. Eng., 26(6), 704 (2008)
Ezeji TC, Karcher PM, Qureshi N, Blaschek HP, Biopro. Biosyst. Eng., 27, 207 (2005)
Qureshi N, Hughes S, Maddox IS, Cotta MA, Biopro. Biosyst. Eng., 27, 215 (2005)
Cho MO, Lee SM, Sang BI, Um YS, KSBB J., 24, 446 (2009)
Qureshi N, Maddox IS, Friedel A, Biotehcnol. Progr., 8, 382 (1992)
Ei-Zanati E, Abdel-Hakim E, El-Ardi O, Fahmy M, J. Membr. Sci., 280(1-2), 278 (2006)
Cho MO, Lee SM, Sang BI, Um YS, KSBB J., 24, 446 (2009)
Choi JM, Yeom SH, Korean J. Chem. Eng., Submitted (2011)
Daugulis AJ, Trends Biotechnol., 19, 459 (2001)
Yeom SH, Daugulis AJ, Process Biochem., 36, 765 (2001)
Littlejohns JV, McAuley KB, Daugulis AJ, J. Hazard. Mater., 175(1-3), 872 (2010)
Yoon JY, Park CH, Kim WJ, Korean J. Biotechnol. Bioeng., 15(4), 380 (2000)
Yang XP, Tsao GT, Biotechnol. Prog., 10(5), 532 (1994)
Yeom SH, Daugulis AJ, Lee SH, Process Biochem., 45, 1582 (2010)
Yeom SH, Daugulis AJ, Biotechnol. Bioeng., 72(2), 156 (2001)
Nielsen DR, Daugulis AJ, McLellan PJ, Biochem. Eng. J., 36, 239 (2007)
Littlejohns JV, McAuley KB, Daugulis AJ, J. Hazard. Mater., 175(1-3), 872 (2010)
Qureshi N, Maddox IS, Friedel A, Biotehcnol. Progr., 8, 382 (1992)
Qureshi N, Blaschek HP, Renew. Eng., 22, 557 (2001)
Ezeji TC, Karcher PM, Qureshi N, Blaschek HP, Biopro. Biosyst. Eng., 27, 207 (2005)