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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received February 16, 2011
Accepted April 24, 2011

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Application of statistical experimental design for optimization of physiological factors and their influences on production of pullulan by Aureobasidium pullulans HP-2001 using an orthogonal array method

Wa Gao^{1 2} Chung-Han Chung^{2 3} Jianhong Li⁴ Jin-Woo Lee^{2 3†}

¹Department of Medical Bioscience, Dong-A University, Busan 604-714, Korea ²BK21 Bio-Silver Project, Dong-A University, Busan 604-714, Korea ³Department of Biotechnology, Dong-A University, Busan 604-714, Korea ⁴College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, Hebei 430070, China

jwlee@dau.ac.kr

Korean Journal of Chemical Engineering, November 2011, 28(11), 2184-2189(6), 10.1007/s11814-011-0107-4

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Abstract

Physiological factors for the production of pullulan by A. pullulans HP-2001 were optimized using orthogonal array method and their influences were compared using Qualitek-4 software. The analysis of variance (ANOVA) indicated that the most important factor for cell growth was yeast extract, whereas that for production of pullulan was glucose. The optimal conditions for cell growth were found to be 100.0 g/L glucose, 10.0 g/L yeast extract, and initial pH of 6.0, whereas those for the production of pullulan were 100.0 g/L glucose, 2.5 g/L yeast extract, and initial pH_x000D_ of 5.5. Among four mineral salts in the medium, potassium phosphate (K2HPO4) was found to be the most important factor for cell growth as well as production of pullulan. Next important salt for cell growth was (NH4)2SO4, whereas that for production of pullulan was NaCl. The optimal concentrations of K2HPO4, NaCl, MgSO4·7H2O, and (NH4)2SO4 for cell growth were 7.5, 1.00, 0.1, and 1.20 g/L, respectively, whereas those for production of pullulan were 2.5, 0.25, 0.8, and 0.30 g/L. The expected cell growth and the production of pullulan by A. pullulans HP-2001 under these optimized conditions were 12.61 and 11.49 g/L, respectively.

Keywords

Pullulan Aureobasidium pullulans Optimization Orthogonal Array Method

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