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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received December 13, 2010
Accepted April 24, 2011

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of three main sugars in cane molasses on the production of butyric acid with Clostridium tyrobutyricum

Lei Huang Yijuan Xiang¹ Jin Cai Ling Jiang Zhengbing Lv¹ Yaozhou Zhang¹ Zhinan Xu^†

Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, P. R. China ¹College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, P. R. China

znxu@zju.edu.cn

Korean Journal of Chemical Engineering, December 2011, 28(12), 2312-2315(4), 10.1007/s11814-011-0110-9

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Abstract

The effects of three main sugars in cane molasses were investigated systematically to prepare a cost-effective medium for butyric acid bioproduction. Additionally, 30 g/L corn steep liquor was screened out as the suitable nitrogen source. In the batch fermentation of free cells, when 60 g/L glucose was the only carbon source, 21.28 g/L butyric acid was achieved after 30 h cultivation. Similar product concentration, productivity and yield were obtained when 60 g/L fructose was applied. The utilization of sucrose would bring about lower productivity (0.29 g/L·h) and product concentration (18.15 g/L), but the yield of butyric acid/sucrose (0.34 g/g) is almost the same as that from glucose or fructose (0.35 g/g). Finally, the sugar mixture (15 g/L glucose, 20g/L fructose and 35 g/L sucrose) was employed to produce butyric acid in a fibrous-bed bioreactor (FBB), and 40.11 g/L butyric acid was produced with one simple fed-batch strategy.

Keywords

Butyric Acid Clostridium tyrobutyricum Cane Molasses Fibrous-bed Bioreactor

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