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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received March 30, 2010
Accepted August 16, 2010

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mutation breeding of nuclease p1 production in Penicillium citrinum by low-energy ion beam implantation

Qinting He Nan Li Xiaochun Chen Qi Ye Jianxin Bai Jian Xiong Hanjie Ying^†

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, P. R. China

Korean Journal of Chemical Engineering, February 2011, 28(2), 544-549(6), 10.1007/s11814-010-0404-3

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Abstract

Nuclease p1 is an important enzyme in the nucleotide industry that is used to hydrolyze nucleic acid into nucleotides. To improve enzyme activity, Penicillium citrinum, a nuclease p1 producing strain, was mutated by lowenergy Nitrogen ion beam implantation at an energy level of 15 keV and a dose ranging from 1×10^(15)-1×10^(16) ions/cm2. The mutant strain designated as N409 was obtained with a high yield of nuclease p1. The activity of nuclease p1 was 421 U/mL from the mutant strain N409, which was increased by 86% compared with the control. The fermentation_x000D_ kinetics of nuclease p1 by the mutant strain N409 was studied in a 30 L external airlifting bioreactor. A model was proposed using the logistic equation for microbial growth, the Luedeking-Piret equation for product formation and a Luedeking-Piret-like equation for substrate uptake. The results predicted from the model were in good agreement with the experimental observations.

Keywords

Ion Beam Implantation Mutation Penicillium citrinum Nuclease p1 Kinetic Model

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