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Received May 2, 2010
Accepted June 20, 2010
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Effect of calcium carbonate in waste office paper on enzymatic hydrolysis efficiency and enhancement procedures
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China 2College of Life Science, Henan Agricultural University, Zhengzhou 450002, China 3Jilin Fuel Alcohol Co., Ltd, PetroChina Corporation, Jilin Economic Development Zone, Jilin 132101, China
jbao@ecust.edu.cn
Korean Journal of Chemical Engineering, February 2011, 28(2), 550-556(7), 10.1007/s11814-010-0365-6
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Abstract
Hydrolysis of waste office paper (WOP) into fermentable sugars is an important option of WOP utilization. In this work, the effect of major chemicals in WOP on its hydrolysis using industrial cellulase Accellerase 1000 (Genencor, Rochester, NY, USA) was investigated, and calcium carbonate (CaCO3) was found to be the key parameter affecting the enzymatic hydrolysis efficiency of WOP. The pretreatment methods, acid washing and acid presoaking, were tested_x000D_
for the removal of CaCO3 from WOP. It was found that the presoaking of sulfuric acid (H2SO4) in WOP was an effective way. The pretreating parameters of WOP were studied on maximizing the hydrolysis efficiency. The conversion yield of cellulose to glucose and cellobiose using the pretreated WOP reached 73.3% after 96 hours hydrolysis at the optimal conditions. The results provided the WOP utilization with a practical enzymatic hydrolysis method with industrial application potential.
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