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Received August 23, 2023
Accepted August 23, 2023
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Expression and purification of beefy meaty peptide in Pichia pastoris
Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Ministry of Education, Tianjin 300457, China 1Beijing Kawin Bio-Tech Co., Ltd, Beijing, China 2Faculty of Sciences, Department of Home and Health Sciences, Allama Iqbal Open University, H-8, Islamabad Pakistan
Korean Journal of Chemical Engineering, March 2011, 28(3), 848-852(5), 10.1007/s11814-010-0439-5
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Abstract
We established a gene expression process to produce a flavor peptide in Pichia pastoris. The octapeptide Lys-Gly-Asp-Glu-Glu-Ser-Leu-Ala was isolated from beef digested by papain, known as “beefy meaty peptide” (BMP). It was considered a savory seasoning in previous literatures. To produce BMP by microorganisms, BMP expression structures encoding tandem repeats of the octapeptide were designed and cloned in E. coli DH5α, then integrated into_x000D_
the AOX1 gene of Pichia pastoris GS115. Clones containing different BMP-gene copies, with 4-copy, 8-copy, 12-copy and 16-copy, were obtained and expressed in Pichia pastoris GS115. For the ease of purification, 6×His tag was fused to the C-termini of the peptides. As a result, the fusion peptides were successfully purified by His-tag bind affinity resin. The BMP fusion peptides with expected sizes were secreted from the resulting strains of P. pastoris GS115.
References
Yamasaki Y, Maekawa KA, Agric. Biol. Chem., 42, 1761 (1978)
Yamasaki Y, Maekawa K, Agric. Biol. Chem., 44, 93 (1980)
Wang K, Maga JA, Bechtel PJ, J. Food Sci., 61, 837 (1996)
Tamura M, Nakatsuka T, Tada M, Kawasaki Y, Kikuchi E, Okai H, Agric. Biol. Chem., 53, 319 (1989)
Wang K, Maga JA, Bechtel PJ, Lebensm.-Wiss Technol., 28, 539 (1995)
Sreekrishna K, Brankamp RG, Kropp KE, Blankenship DT, Tsay JT, Smith PL, Wierschke JD, Subramaniam A, Birkenberger LA, Gene., 190, 55 (1997)
Zhao X, Huo K, Li Y, Chinese J. Biotechnol., 16, 308 (2000)
Schagger H, von Jagow G, Anal. Chem., 166, 368 (1987)
Ellis SB, Brust PF, Koutz PJ, Waters AF, Harpold MM, Gingeras TR, Mol. Cell. Biol., 5, 1111 (1985)
Cregg JM, Barringer KJ, Hessler AY, Madden KR, Mol. Cell. Blol., 5, 3376 (1985)
Zhong XF, Peng LS, Zheng SL, Sun ZZ, Ren YF, Dong ML, Xu AL, Protein Expr. Purif., 36(2), 165 (2004)
Xie JL, Zhang L, Ye Q, Zhou QW, Xin L, Du P, Gan RB, Biotechnol. Lett., 25(2), 173 (2003)
Chung HJ, Park SM, Kim HR, Yang MS, Kim DH, Enzyme Microb. Technol., 31(4), 384 (2002)
Nakata T, Takahashi M, Nakatani M, Kuramitsu R, Tamura M, Okai H, Japan Society for Bioscience, Biotechnology, and Agrichemistry., 59, 689 (1995)
Moralejo FJ, Cardoza RE, Gutierrez S, Martin JF, Appl. Environ. Microbiol., 65, 1168 (1999)
Kondo K, Miura Y, Sone H, Kobayashi K, Iijima H, Nat. Biotechnol., 15, 453 (1997)
Yamasaki Y, Maekawa K, Agric. Biol. Chem., 44, 93 (1980)
Wang K, Maga JA, Bechtel PJ, J. Food Sci., 61, 837 (1996)
Tamura M, Nakatsuka T, Tada M, Kawasaki Y, Kikuchi E, Okai H, Agric. Biol. Chem., 53, 319 (1989)
Wang K, Maga JA, Bechtel PJ, Lebensm.-Wiss Technol., 28, 539 (1995)
Sreekrishna K, Brankamp RG, Kropp KE, Blankenship DT, Tsay JT, Smith PL, Wierschke JD, Subramaniam A, Birkenberger LA, Gene., 190, 55 (1997)
Zhao X, Huo K, Li Y, Chinese J. Biotechnol., 16, 308 (2000)
Schagger H, von Jagow G, Anal. Chem., 166, 368 (1987)
Ellis SB, Brust PF, Koutz PJ, Waters AF, Harpold MM, Gingeras TR, Mol. Cell. Biol., 5, 1111 (1985)
Cregg JM, Barringer KJ, Hessler AY, Madden KR, Mol. Cell. Blol., 5, 3376 (1985)
Zhong XF, Peng LS, Zheng SL, Sun ZZ, Ren YF, Dong ML, Xu AL, Protein Expr. Purif., 36(2), 165 (2004)
Xie JL, Zhang L, Ye Q, Zhou QW, Xin L, Du P, Gan RB, Biotechnol. Lett., 25(2), 173 (2003)
Chung HJ, Park SM, Kim HR, Yang MS, Kim DH, Enzyme Microb. Technol., 31(4), 384 (2002)
Nakata T, Takahashi M, Nakatani M, Kuramitsu R, Tamura M, Okai H, Japan Society for Bioscience, Biotechnology, and Agrichemistry., 59, 689 (1995)
Moralejo FJ, Cardoza RE, Gutierrez S, Martin JF, Appl. Environ. Microbiol., 65, 1168 (1999)
Kondo K, Miura Y, Sone H, Kobayashi K, Iijima H, Nat. Biotechnol., 15, 453 (1997)
