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Received August 23, 2023
Accepted August 23, 2023
articles This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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The role of environmental factors and medium composition on bacteriocin production by an aquaculture probiotic Enterococcus faecium MC13 isolated from fish intestine

Department of Biotechnology, School of Life Sciences, Pondicherry University, Pondicherry-605014, India
varul18@gmail.com
Korean Journal of Chemical Engineering, March 2011, 28(3), 860-866(7), 10.1007/s11814-010-0443-9
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Abstract

The aim of this study was to optimize medium composition for higher yield of total viable cells and bacteriocin by Enterococcus faecium MC13. The factors such as peptone, meat extract, yeast extract, lactose, glycerol, tween 80, triammonium citrate and K2HPO4 were selected based on the Lactobacillus MRS medium composition. Two level factorial designs (FD) and steepest ascent path were performed to identify vital factors among the variables. Through the 2.8_x000D_ FD, peptone, yeast extract and lactose were found to be significant factors involved in the enhanced production of viable cells and bacteriocin. Therefore, these three foremost factors were further optimized by central composite design to achieve efficient yield. The optimum MRS composition was found to be peptone (40.0 g/L), meat extract (30.0 g/L), yeast extract (40.0 g/L), lactose (24.0 g/L), glycerol (5.8 g/L), Tween 80 (3.0 g/L), triammonium citrate (1.0 g/L), K2HPO4 (2.5 g/L), MgSO4·7H2O (0.10 g/L), MnSO4·7H2O (0.05 g/L) and dipotassium PO4 (2.0 g/L). The optimized growth medium allowed higher amount of bacteriocin activity (36,100 AUml^(-1)) and total viable cells (14.22 LogCFUml^(-1)) production which were two-times higher than the commercial MRS medium.

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