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Received August 23, 2023
Accepted August 23, 2023
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The role of environmental factors and medium composition on bacteriocin production by an aquaculture probiotic Enterococcus faecium MC13 isolated from fish intestine
Paulraj Kanmani
Ramraj Satishkumar
Neelakandan Yuvaraj
Kupusamy Alagesan Paari
Vellaiyan Pattukumar
Venkatesan Arul†
Department of Biotechnology, School of Life Sciences, Pondicherry University, Pondicherry-605014, India
varul18@gmail.com
Korean Journal of Chemical Engineering, March 2011, 28(3), 860-866(7), 10.1007/s11814-010-0443-9
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Abstract
The aim of this study was to optimize medium composition for higher yield of total viable cells and bacteriocin by Enterococcus faecium MC13. The factors such as peptone, meat extract, yeast extract, lactose, glycerol, tween 80, triammonium citrate and K2HPO4 were selected based on the Lactobacillus MRS medium composition. Two level factorial designs (FD) and steepest ascent path were performed to identify vital factors among the variables. Through the 2.8_x000D_
FD, peptone, yeast extract and lactose were found to be significant factors involved in the enhanced production of viable cells and bacteriocin. Therefore, these three foremost factors were further optimized by central composite design to achieve efficient yield. The optimum MRS composition was found to be peptone (40.0 g/L), meat extract (30.0 g/L), yeast extract (40.0 g/L), lactose (24.0 g/L), glycerol (5.8 g/L), Tween 80 (3.0 g/L), triammonium citrate (1.0 g/L), K2HPO4 (2.5 g/L), MgSO4·7H2O (0.10 g/L), MnSO4·7H2O (0.05 g/L) and dipotassium PO4 (2.0 g/L). The optimized growth medium allowed higher amount of bacteriocin activity (36,100 AUml^(-1)) and total viable cells (14.22 LogCFUml^(-1)) production which were two-times higher than the commercial MRS medium.
Keywords
References
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Swain SM, Chandrasekar S, Arul V, World J. Microbiol. Biotechnol., 25, 697 (2009)
Todorov SD, Dicks LMT, Enzym. Microb. Technol., 36, 326 (2005)
Prema P, Bharathy S, Palavesam M, Sivasubramanian M, Immanuel G, World J. Microb. Biotechnol., 22, 865 (2006)
Kabuki T, Uenishi H, Watanabe M, Seto Y, Nakajima H, J. Appl. Microbiol., 102(4), 971 (2007)
Todorov SD, Dicks LMT, Braz. J. Microb., 38, 166 (2007)
Abada EAE, Animal Cell. Systm., 12, 41 (2008)
Delgado A, Lopez FNA, Brito D, Peres C, Fevereiro P, Garrido-Fernandez A, J. Biotechnol., 130, 193 (2007)
Delgado A, Brito D, Peres C, Arroyo-Lopez FN, Garrido-Fernandez A, Food Microb., 22, 521 (2005)
Preetha R, Jeyaprakash NS, Philp R, Bright sigh IS, Bitechnol. Biopro. Eng., 12, 548 (2007)
Kayalvizhi N, Gunasekaran P, Lett. Appl. Microb., 47, 600 (2008)
Cho JH, Kim YP, Kim EK, Korean J. Chem. Eng., 26(3), 759 (2009)
Cladera-Olivera F, Caron GR, Brandelli A, Biochem. Eng. J., 21, 53 (2004)
Jimenez-Diaz R, Rios-Sanchez RM, Desmazeaud M, Ruiz-Barba JL, Piard JC, Appl. Environ. Microb., 59, 1416 (1993)
Pattnaik P, Kaushik JK, Grover S, Batish VK, J. Appl. Microbiol., 91(4), 636 (2001)
Cheigh CI, Choi HJ, Park H, Kim SB, Kook MC, Kim TS, Hwang JK, Pyun YT, J. Biotechnol., 95, 225 (2002)
Rodrigues L, Teixeira J, Oliveira R, Van der Mei HC, Process Biochem., 40, 1 (2006)
Marekova M, Laukova A, Skaugen M, Nes I, Ind. Microb. Biotechnol., 34, 537 (2007)