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Received November 8, 2010
Accepted December 27, 2010
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Comparison of methods for preventing methanol inhibition in enzymatic production of biodiesel
Department of Biochemical Engineering, Gangneung-Wonju National University, Gangneung, Gangwon-do 210-702, Korea
Korean Journal of Chemical Engineering, June 2011, 28(6), 1420-1426(7), 10.1007/s11814-010-0526-7
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Abstract
During the enzymatic production of biodiesel, methanol has a major inhibitory effect on enzyme activity whereas glycerol has a minor effect. Revitalization of the methanol-deactivated enzyme or pre-incubation of enzyme with various chemicals turned out to be unsuccessful. The stepwise feeding of methanol, a widely used conventional method for preventing methanol inhibition, was optimized in terms of the aliquot number and feeding interval to obtain a high conversion rate as well as a high degree of final biodiesel conversion. The use of six feedings of methanol with_x000D_
an equivalent molar ratio of 0.75 at 3-h intervals was found to be the optimal mode for preventing methanol inhibition; a biodiesel conversion rate of approximately 95% could be achieved within 20 h by using this method. Finally, to prevent contact between the undissolved methanol and the enzyme, methanol was pre-dissolved in water or biodiesel and fed to the mixture of soybean oil and the enzyme. This pre-dissolution method completely prevented enzyme inhibition, and a final biodiesel conversion rate of 82.3% was obtained.
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