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Received December 19, 2010
Accepted April 24, 2011
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A study of mycelial growth and exopolysaccharide production from a submerged culture of Mycoleptodonoides aitchisonii in an air-lift bioreactor
DuBok Choi
Jun Han Lee1
Yun-Soo Kim1
Myung-Sun Na2
On-You Choi3
Hee-Duck Lee4
Myung Koo Lee
Wol-Suk Cha1†
Department of Pharmacy, College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea 1Department of Biochemical and Chemical Engineering, Chosun University, Gwangju 501-759, Korea 2Department of Beauty and Cosmetic, Chosun University, Gwangju 501-759, Korea 3Department of Environmental Health, Cho-dang University, Jeonnam 534-800, Korea 4Korea Advanced Food Research Institute, Seoul 137-850, Korea
wscha@mail.chosun.ac.kr
Korean Journal of Chemical Engineering, June 2011, 28(6), 1427-1432(6), 10.1007/s11814-011-0109-2
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Abstract
We determined the optimal culture and medium conditions for effective production of mycelial mass and exopolysaccharide from a liquid culture of Mycoleptodonoides aitchisonii in an air-lift bioreactor. The mycelial growth and exopolysaccharide production were found to be optimal at a temperature of 25 ℃ and pH of 6.5. When 60 g/L of lactose was used as a carbon source, the maximum mycelial growth and exopolysaccharide production were obtained. The polypeptone and yeast extract were the most appropriate nitrogen sources for mycelial growth and exopolysaccharide production. In addition, when a mixture of 20 g/L of polypeptone and 5 g/L of yeast extract was used, the exopolysaccharide production increased 50% compared to that of the sole nitrogen source. CaCl2·2H2O (1.0 g/L) was the most effective mineral source. Using the optimal culture and medium conditions, batch cultures with basal and designed medium on mycelial growth and exopolysaccharide production in a 5 L air-lift bioreactor were carried out for 16 days. The mycelial growth and exopolysaccharide production increased with an increase of culture time at 14 days, and the maximum mycelial growth and exopolysaccharide production were 20.3 and 6.2 g/L, respectively, after 14 days of culture. The developed model in an air-lift bioreactor showed good agreement with experimental data. These results indicate that exopolysaccharide production is associated with the mycelial growth of M. aitchisonii in an air-lift bioreactor.
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Choi JH, Horikawa M, Okumura H, Kodani S, Nagai K, Hashizume D, Koshino H, Kawagishi H, Tetrahedron., 65, 221 (2009)
Cha WS, Lee MY, Cho BS, Park SY, Oh DG, Korean J. Life Sci., 14, 560 (2004)
Choi DB, Nam HG, Cha WS, Korean J. Chem. Eng., 23(2), 241 (2006)
Choi DB, Kang SY, Song YH, Kwun KH, Jeong KJ, Cha WS, J. Microbiol. Biotechnol., 15, 368 (2005)
Shin SE, Cha WS, Kang SH, Korean J. Life Sci., 13, 492 (2003)
Choi DB, Cho KA, Cha WS, Biotechnol. Bioprocess Eng., 9, 171 (2004)
Kim DH, Yang BK, Jeong SC, Park JB, Cho SP, Das S, Yun JW, Song CH, Biotechnol. Lett., 23, 513 (2004)
Zadrazil F, Mush. Sci., 9, 621 (1974)
Kim JM, Ra KS, Noh DO, Suh HJ, J. Ind. Microbiol. Biotechnol., 29, 292 (2002)
Kim HM, Park SY, Ra KS, Koo KB, Yun JW, Choi JW, J. Microbiol., 44, 233 (2006)
Park JP, Kim SW, Hwang HJ, Yun JW, Lett. Appl. Microbiol., 33, 76 (2001)
Lee JS, Wee JW, Lee HY, An HS, Hong EK, Biotechnol. Bioprocess Eng., 15, 453 (2010)
Papinutti L, Forchiassin F, Biotechnol. Bioprocess Eng., 15, 1102 (2010)
Fang QH, Zhong JJ, Pro. Biochem., 37, 769 (2002)
Lee KM, Lee SY, Lee HY, J. Biosci. Bioeng., 88(6), 646 (1999)
Xiao JH, Chen DX, Liu JW, Liu ZL, Wan WH, Fang N, Xiao Y, Qi Y, Ling ZQ, J. Appl. Microbiol., 96, 1105 (2005)
Kim YK, Xiao CL, Rogers JD, Mycologia., 97, 25 (2005)
Kim YR, Korean Soc. Mycol., 31, 205 (2003)
Park KS, Lee JD, Korean J. Biotechnol. Bioeng., 6, 91 (1991)
Kim HK, Park JS, Cha DY, Kim YS, Moon BJ, Korean J. Mycol., 22, 145 (1994)
Chang HY, Cha DY, Kang AS, Hong IP, Kim KP, Seok SJ, Ryu YJ, Sung JM, Korean J. Mycol., 23, 238 (1995)
Hashimoto K, Takahashi Z, Mush. Sci., 6, 585 (1974)
Hong JS, Lee KS, Choi DS, Korean J. Mycol., 9, 19 (1981)
Hong IP, Lee MW, Korean J. Mycol., 18, 42 (1990)
Chi JH, Ha TM, Kim YH, Rho YD, Korean J. Mycol., 24, 214 (1996)
Kim HK, Cheong JC, Chang HY, Kim GP, Cha DY, Moon BJ, Korean J. Mycol., 25, 305 (1997)
Wol-port FS, Ann. Misouri Bot. Garden., 1, 11 (1924)
Chang ST, Miles PG, Edible mushrooms and their cultivations, CRC Press Inc., Boca Raton, Florida (1989)
Hogberg N, Holdenrieder O, Jan S, Heredity., 83, 354 (1999)
Jonathan SG, Fasidi IO, Food Chem., 75, 303 (2001)
Cui F, Liu Z, Li Y, Ping L, Ping L, Zhang Z, Lin L, Dong Y, Huang D, Biotechnol. Bioprocess Eng., 15, 299 (2010)
Kim SS, Lee JS, Cho JY, Kim YE, Hong EK, Biotechnol. Bioprocess Eng., 15, 293 (2010)
Bae JT, Sinha J, Park JP, Song CH, Yun JW, J. Microbiol. Biotechnol., 10, 482 (2000)
Manzoni M, Rollini M, Biotechnol. Lett., 23(18), 1491 (2001)
Bae JT, Park JP, Song CH, Yu CB, Park MK, Yun JW, J. Biosci. Bioeng., 91(5), 522 (2001)
Chatterjee R, Majumder K, Sengupta S, Biotechnol. Bioprocess Eng., 15, 854 (2010)
Magasanik B, Prog. Nucelic Acid Res. Mol. Biol., 17, 99 (1976)
Park KS, Park S, Jung IC, Ha HC, Kim SH, Lee JS, Korean J. Mycol., 22, 184 (1994)
Lee SY, Kang TS, Korean J. Appl. Microbiol. Biotechnol., 24, 111 (1996)
Jonathan SG, Fasidi IO, Food Chem., 75, 303 (2001)
Kang TS, Lee DG, Lee SY, Korean J. Mycol., 25, 257 (1977)
Katoh T, Yuguchi D, Yoshii H, Shi H, Shimizu K, J. Biotechnol., 67, 113 (1999)
MOHAMMAD FHA, BADRELDIN SM, ELTAYEB OM, ELRAHMAN OAA, Biomass Bioenerg., 8(2), 121 (1995)
Tang YJ, Zhong JJ, Biochem. Eng. J., 21, 259 (2004)
Choi DB, Kang SY, Song YH, Kwun KH, Jeong KJ, Cha WS, J. Microbiol. Biotechnol., 15, 368 (2005)
