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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received May 26, 2010
Accepted December 28, 2010

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Studies on growth kinetics of predominantly Pseudomonas sp. in internal loop airlift bioreactor using phenol and m-cresol

Pichiah Saravanan^† Kannan Pakshirajan¹ Prabirkumar Saha²

Water Research Centre, Department of Civil Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia ¹Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, India ²Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, India

pichiahsaravanan@gmail.com

Korean Journal of Chemical Engineering, July 2011, 28(7), 1550-1555(6), 10.1007/s11814-010-0531-x

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Abstract

Growth profile of predominantly Pseudomonas species was studied using wastewater containing phenol and m-cresol, as single and multi component systems in an internal loop airlift bioreactor (ILALR). The species utilized for the study was isolated from a wastewater treatment plant. The reactor was operated at both lower and higher hydraulic retention time (HRTs), 4.1 h and 8.3 h, respectively. The inlet phenol and concentration was varied between 100 and 800 mg/L with 800 mg/L as shock loading concentration for an HRT of 8.3 h. For 4.1 h HRT, the concentration was_x000D_ varied 100 and 500 mg/L using 500 mg/L as a shock loading concentration. The study showed complete degradation of both phenol and m-cresol, when present individually at an HRT of 8.3 h with an enriched biomass output. The specific growth rate of the culture at various phenol and m-cresol concentrations was fitted to a Monod model. The biokinetics value showed good potential of Pseudomonas species employing the internal loop air lift bioreactor in utilizing high strength phenolics containing wastewater. Culture growth profile with both phenol and m-cresol as mixtures also showed decreased lag times with complete utilization of the phenolics.

Keywords

Biomass Internal Loop Airlift Bioreactor Monod Model Phenolics Pseudomonas sp. Wastewater

References

Lefrancois L, Mariller CGT, France JV, No. 1,102,200, Delivree le 4 Mai, French (1955)
Kanai T, Uzumaki T, Kawase Y, Comput. Chem. Eng., 20(9), 1099 (1996)
Couvert A, Bastoul D, Roustan M, Chatellier P, Chem. Eng. Process., 43(11), 1381 (2004)
Quan XC, Shi HC, Zhang YM, Wang HL, Qian Y, Sep. Purif. Technol., 34(1-3), 97 (2004)
Viggiani A, Olivieri G, Siani L, Di Donato A, Marzocchella A, Salatino P, Barbieri P, Galli E, J. Biotechnol., 123(4), 464 (2006)
Jajuee B, Margaritis A, Karamanev D, Bergougnou MA, Biotechnol. Bioeng., 96(2), 232 (2007)
Feng W, Wen JP, Liu CY, Yuan Q, Jia XQ, Sun Y, Biotechnol. Bioeng., 97(2), 251 (2007)
Kumar A, Kumar S, Kumar S, Biochem. Eng. J., 22(2), 151 (2005)
Juang RS, Tsai SY, Biochem. Eng. J., 31(2), 133 (2006)
Abuhamed T, Bayraktar E, Mehmetoglu T, Mehmetoglu U, Process. Biochem., 39(8), 983 (2004)
Monteiro AAMG, Boaventura RAR, Rodrigues AE, Biochem. Eng. J., 6(1), 45 (2000)
Yeom SH, Kim SH, Yoo YJ, In SY, Korean J. Chem. Eng., 14(1), 37 (1997)
Zhao Z, Jiang G, Jiang S, Ding F, Korean J. Chem. Eng., 26(6), 1662 (2009)
Vinod VA, Reddy GV, Biochem. Eng. J., 24(1), 1 (2005)
Saravanan P, Pakshirajan K, Saha, J. Environ. Sci., 20(12), 1508 (2008)
Saravanan P, Pakshirajan K, Saha P, Int. J. Environ. Eng., 2, 303 (2010)
Monod J, Annu. Rev. Microbiol., 3, 371 (1949)