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Received September 7, 2010
Accepted March 22, 2011
- This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Effects of bmim[PF6] treatments with different concentrations on microbial activity of Saccharomyces cerevisiae
College of Food Science, South China Agricultural University, Guangzhou 510642, China
Korean Journal of Chemical Engineering, September 2011, 28(9), 1902-1907(6), 10.1007/s11814-011-0076-7
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Abstract
To study the role of ionic liquid as a solvent in whole cell biocatalyst, it is necessary to probe the effects of ionic liquid treatments on microbial activity. In this paper, Saccharomyces cerevisiae was selected as a test bacterium to study the effects of bmim[PF6] ionic liquid in different concentration on yeast activity through determining the growth curve, sugar degradation curve, microbial activity, colonial morphology and cell morphology. The results revealed that the growth of yeast is inhibited strongly in low concentration ionic liquid, while the lethal effect is feeble in high concentration ionic liquid. This result was totally different from that of supercritical CO2 treatment or high concentration benzene methanol treatment which would lead most yeast to death.
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References
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McEwen AB, Ngo HL, LeCompte K, Goldman JL, J. Electrochem. Soc., 146(5), 1687 (1999)
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Kaar JL, Jesionowski AM, Berberich JA, Moulton R, Russell AJ, J. Am. Chem. Soc., 125(14), 4125 (2003)
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Tee KL, Roccatano D, Stolte S, Arning J, Bernd J, Schwaneberg U, Green Chem., 10(1), 117 (2008)
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Sang HL, Sung HH, Hiep NM, Chang WJ, Koo YM, J. Biotechnol., 133, 486 (2008)
Naik PU, Nara SJ, Harjani JR, Salunkhe MM, J. Mol. Catal. B: Enzym., 44(3-4), 93 (2007)
Matsuda T, Yamagishi Y, Koguchi S, Lwai N, Kitazume T, Tetrahedron. Lett., 37(41), 4617 (2006)
Brautigam S, Bringer-meyer S, Tetrahedron: Asymmetry., 18(6), 1883 (2007)
Wang W, Zong MH, Lou WY, J. Mol. Catal. B: Enzym., 56, 70 (2009)
He L, Laboratory Technology in Food Microbes, Beijing: China Agricultural University Press, 78 (2001)
Zhuge J, Wang ZX, Industrial Microbiology Technical Manual, Beijing: China Light Industry Press (1994)
Lee SM, Chang WJ, Choi AR, Koo YM, Korean J. Chem. Eng., 22(5), 687 (2005)
Ganske F, Bornscheuer UT, Biotechnol. Lett., 28(7), 465 (2006)