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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received May 12, 2011
Accepted June 7, 2011

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comparison of inhibition effects of various isolated lignins on enzymatic hydrolysis of cellulose

Tae Hyun Kim^{1 2†}

¹Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, United States, USA ²Department of Natural Resource Ecology and Management, Iowa State University, Ames, IA 50011, United States, USA

thkim@iastate.edu

Korean Journal of Chemical Engineering, January 2012, 29(1), 82-88(7), 10.1007/s11814-011-0150-1

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Abstract

Various isolated soluble and insoluble lignins isolated from ammonia recycle percolation (ARP), dilute sulfuric acid, and hot-water pretreatments and two commercially available lignins (alkali and organo-solv lignin) were tested to evaluate their inhibitory effects on the enzymatic hydrolysis of cellulose. The purpose of this study was to compare the effects by adding various isolated lignins in the enzyme hydrolysis. The results showed that enzyme reaction was generally inhibited by addition of lignins. The order of lignins from most to least inhibitory effects were alkali, ARP-soluble, water-soluble, acid-soluble, ARP-insoluble, and organo-solv lignins. With 0.344 g ARP-soluble or alkali lignin/g-glucan lignin loadings, 72-h digestibilities were decreased by 16% and 20%, respectively. It was also observed that the digestibilities were decreased, as the lignin loadings increased. The hindering effect by ARP-insoluble or organosolv_x000D_ lignin was not significant.

Keywords

Enzyme Inhibition Pretreatment Alkali Lignin Water Soluble Lignin Acid Soluble Lignin Organo-solv Lignin

References

Chang VS, Holtzapple MT, Appl. Biochem. Biotechnol., 84/86, 5 (2000)
Cowling EB, Kirk TK, Biotechnol. Bioeng. Symp., 6, 95 (1976)
Dulap CE, Thomson J, Chiang LC, Symp. Ser., 72(158), 58 (1976)
Lee D, Yu AH, Saddler JN, Biotechnol. Bioeng., 45(4), 328 (1995)
Mooney CA, Mansfield SD, Touhy MG, Saddler JN, Bioresour. Technol., 64(2), 113 (1998)
Schwald W, Brownell HH, Saddler JN, J. Wood Chem. Technol., 8(4), 543 (1988)
Agosin E, Monties B, Odier E, J. Sci. Food Agric., 36, 925 (1985)
Chesson A, Gordon AH, Lomax JA, J. Agri. Sci. Camb., 34, 1330 (1983)
Berlin A, Gilkes N, Kurabi A, Bura R, Tu M, Kilburn D, Saddler JN, Appl. Biochem. Biotechnol., 121-124, 163 (2005)
Garrote G, Dominguez H, Parajo JC, J. Food Eng., 52, 211 (2002)
Lora JH, Wayman M, Tappi., 61, 47 (1978)
Vaquez MJ, Alonso JL, Dominguesz H, Parajo JC, World J. Microbiol. Biotechnol., 17, 817 (2001)
Allen SG, Schulman D, Lichwa J, Antal MJ, Laser M, Lynd LR, Ind. Eng. Chem. Res., 40(13), 2934 (2001)
Mok WS, Antal MJ, Varhegyi G, Ind. Eng. Chem. Res., 31(1), 94 (1992)
Kim JS, Lee YY, Torget RW, Appl. Biochem. Biotechnol., 91-93, 331 (2001)
Grethlein H, Nature Biotechnol., 3, 155 (1985)
Burns DS, Oshima H, Converse AO, Appl. Biochem. Biotechnol., 24-25(1), 67 (1990)
Jacobsen SE, Wyman CE, Appl. Biochem. Biotechnol., 84-86, 81 (2000)
Tarkow F, Feist WC, A mechanism for improving the digestibility of lignocellulosic materials with dilute alkali and liquid ammonia, In: Hajny, G. J., Reese, E.T. Ed. Cellulases and their applications, Washington DC: American Chemical Society (1969)
Yoon HH, Wu ZW, Lee YY, Appl. Biochem. Biotechnol., 51/52, 5 (1995)
Kim SB, Lee YY, Appl. Biochem. Biotechnol., 57/58, 147 (1996)
Ferrer A, Byers FM, Sulbaran-de-Ferrer B, Dale BE, Aiello C, Appl. Biochem. Biotechnol., 84-86, 163 (2000)
Iyer PW, Wu ZW, Kim SB, Lee YY, Appl. Biochem. Biotechnol., 57/58, 121 (1996)
Li X, Kim TH, Bioresour. Technol., 102, 4779 (2011)
Kim TH, Kim JS, Sunwoo C, Lee YY, Bioresour. Technol., 90(1), 39 (2003)
Kim TH, Taylor F, Hicks KB, Bioresour. Technol., 99(13), 5694 (2008)
Kim TH, Lee YY, Appl. Biochem. Biotechnol., 137-140(112), 81 (2007)
Kim TH, Lee YY, Sunwoo C, Kim JS, Appl. Biochem. Biotechnol., 133(1), 41 (2006)
Kim TH, Lee YY, Bioresour. Technol., 96(18), 2007 (2005)
Kim TH, Lee YY, Appl. Biochem. Biotechnol., 124(1-3), 1119 (2005)
He YF, Pang YZ, Liu YP, Li XJ, Wang KS, Energy Fuels, 22(4), 2775 (2008)
Nakagame S, Chandra RP, Kadla JF, Saddler JN, Biotechnol. Bioeng., 108(3), 538 (2011)
Kawamoto H, Nakatsubo F, Murakami K, Mokuzai Gakkaishi., 38, 81 (1992)
Sewalt VJH, Glassner WG, Beauchemin KA, J. Agric.Food Chem., 45, 1823 (1997)
Kumar R, Mago G, Balan V, Wyman CE, Bioresour. Technol., 100, 3948 (2009)
Qing Q, Yang B, Wyman CE, Bioresour. Technol., 101, 9624 (2010)
NREL, Chemical Analysis and Testing Laboratory Analytical Procedures (CAT), National Renewable Energy Laboratory, Golden, Golden, CO. (2008)
Chum HL, L. J. Douglas, D. A. Feinberg and H. A. Schroeder, Evaluation of pretreatments of biomass for enzymatic hydrolysis of cellulose, SERI/TP-231-2183, SERI, Golden, CO. (1985)
Grohmann K, Torget RW, Himmel ME, Biotechnol. Bioeng. Symp., 15, 59 (1985)