The composition and phase diagram of aqueous two-phase systems (ATPSs) of urethanized polyvinyl alcohol (UPVA) and a series of ionic co- and terpolymers (IPs) of pH-responsive hydrophilic diallylammonioethanoate, 0-3mol% hydrophobic dodecyldiallylammonium chloride, and SO2 have been studied for the first time. The presence of unquenched nitrogen valency in the repeat units of the IPs permitted the change of the charge types and their densities in the polymer chain. The effects of zwitterionic (Z±) and anionic (A-) charge densities on the polymer backbone, salt (NaCl) concentration, and hydrophobe content on the phase diagrams were investigated. The presence of the hydrophobe in the polymer shifted the binodal downward; magnitude of the shift increased with the increase in the hydrophobe content. The phase separation happened at relatively low total polymer concentrations (much below than 10%), which could be useful in separation and purification of biomaterials.

Aqueous Two-phase System pH-responsive Polymers Hydrophobicity Urethanized Polyvinyl Alcohol Phase Diagram

Johansson HO, Magaldi FM, Feitosa E, Pessoa A, J.Chromatogr. A., 1178, 153 (2008)
Benavides J, Aguilar O, Lapizco-Encinas BH, Rito-Palomares M, Chem. Eng. Technol., 31(6), 838 (2008)
Duarte SP, Fortes AG, Prazeres DMF, Marcos JC, J.Chromatogr. A., 1164, 105 (2007)
Salabat A, Abnosi MH, Bahar AR, J. Chromatogr. B., 858, 234 (2007)
Bora MM, Borthakur S, Rao PC, Dutta NN, Sep. Purif. Technol., 45(2), 153 (2005)
Andrews BA, Schmidt AS, Asenjo JA, Biotechnol. Bioeng., 90(3), 380 (2005)
Su CK, Chiang BH, Process Biochem., 41, 257 (2005)
Walter H, Brookes DE, Fisher D, Partitioning in Aqueous Two-Phase systems: Theory, Methods, Uses and Application to Biotechnology, Academic Press, Orlando (1989)
Albertsson PA, Partition of cell particle and macromolecules, John Wiley, New York (1986)
Albertsson PA, Tjerneld F, Methods Enzymol., 228, 3 (1994)
Dissing U, Mattiasson B, Biotechnol. Appl. Biochem., 17, 15 (1993)
Bergfelt K, Piculell L, Tjerneld F, Macromolecules, 28(9), 3360 (1995)
Svensson M, Linse P, Tjerneld F, Macromolecules, 28(10), 3597 (1995)
Lu M, Albertsson PA, Johansson G, Tjerneld F, J. Chromatogr. A., 668, 215 (1994)
Sivars U, Bergfeldt K, Pictulell L, Tjerneld F, J. Chromatogr. B., 680, 43 (1996)
Birkenmeier G, Kunath M, J. Chromatogr. B., 680, 97 (1996)
Lu M, Tjerneld F, J. Chromatogr. A., 766, 99 (1997)
Chung BH, Kim WK, Song KB, Kim CH, Rees SK, Biotechnol. Technol., 11, 327 (1997)
Huaxue Shijie., 240-242, 251, 48 (2007)
Li Z, Pei Y, Wang H, Fan J, Wang J, TrAC, Trends in Analytical Chemistry., 29, 1336 (2010)
Hamad EZ, Ijaz W, Ali SA, Hastaoglu MA, Biotechnol. Prog., 12(2), 173 (1996)
Waziri SM, Abu-Sharkh BF, Ali SA, Fluid Phase Equilib., 205(2), 275 (2003)
Waziri SM, Abu-Sharkh BF, Ali SA, Biotechnol. Prog., 20(2), 526 (2004)
Andrews BA, Asenjo JA, Sep. Sci. Technol., 45(15), 2165 (2010)
Sakurada I, Nakajima A, Shibatani K, J. Polym. Sci., Part A., 3545 (1964)
Umar Y, Al-Muallem HA, Abu-Sharkh BF, Ali SA, Polymer, 45(11), 3651 (2004)
Ali SA, Ahmed SZ, Wazeer MI, Hamad EZ, Polymer, 38(13), 3385 (1997)
Al-Muallem HA, Wazeer MIM, Ali SA, Polymer, 43(4), 1041 (2002)
Munk P, Introduction to Macromolecular Science, Wiley/Interscience, New York (1989)
Saito S, J. Polym. Sci. Poly. Chem. Ed., 7, 1789 (1969)
Diamond AD, Hsu JT, Correlation of polymer partitioning in aqueous two-phase systems, AIChE symposium series 88, (#290), 105 (1992)