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Received September 5, 2011
Accepted April 27, 2012
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Biological synthesis of gold nanoparticles by Bacillus subtilis and evaluation of increased antimicrobial activity against clinical isolates
Department of Biotechnology, P. R. Engineering College, Thanjavur 613 403, India 1Department of Energy Engineering, Kumaraguru College of Technology, Coimbatotore 641 049, India 2Department of Biotechnology, Karunya University, Coimbatore 641 114, India
biotechthiru@gmail.com
Korean Journal of Chemical Engineering, December 2012, 29(12), 1761-1765(5), 10.1007/s11814-012-0055-7
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Abstract
Biological sources of microorganisms and plants are playing a major role in the reduction of metallic nanoparticles such as silver and gold, as it emerges as an eco-friendly and exciting approach in nanotechnology. We report on the biological synthesis of gold nanoparticles using the culture supernatant of Bacillus subtilis and its effect on increased antibacterial and antifungal activities against clinically isolated organism. When the supernatant of Bacillus subtilis was added to HAuCl4 aqueous solution, HAuCl4 was reduced as Au+ ions, which confirmed the presence of nanoparticles by the color change of pale yellow to purple. The minimum and maximum peaks were observed at 24th and 120th hours by UV-Visible spectroscopy. The combined antibacterial and antifungal activities with various antibiotics were observed against clinical isolates.
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