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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received November 5, 2011
Accepted May 25, 2012

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A new electrochemical biosensor for hydrogen peroxide using HRP/AgNPs/cysteamine/p-ABSA/GCE self-assembly modified electrode

Ali Shokuhi Rad^† Mohsen Jahanshahi¹ Mehdi Ardjmand² Ali-Akbar Safekordi³

Chemical Engineering Group, Faculty of Engineering, Qaemshahr Branch, Islamic Azad University, Tehran, Iran ¹Nanotechnology Research Institute, School of Chemical Engineering, Babol University of Technology, Babol, Iran ²Chemical Engineering Group, Faculty of Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran ³Chemical Engineering Group, Faculty of Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

A.Shokuhi@gmail.com

Korean Journal of Chemical Engineering, December 2012, 29(12), 1766-1770(5), 10.1007/s11814-012-0078-0

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Abstract

An electrochemical hydrogen peroxide biosensor was designed by immobilizing horseradish peroxidase (HRP) on Ag nanoparticles/cysteamine/p-aminobenzene sulfonic acid/glassy carbon (GC) electrode. Ag nanoparticles can act as tiny conduction centers on electrodes that adsorb redox enzymes, facilitating the transfer of electrons with no requiring any loss of biological activity. The forerunner film was first electropolymerized on the glassy carbon electrode with p-aminobenzene sulfonic acid (p-ABSA) by cyclic voltammetry. The cysteamine (CA) was bound on the surface of the film by electrostatic force, then Ag nanoparticles were immobilized on the cysteamine monolayer, and lastly HRP was adsorbed onto the surfaces of the Ag nanoparticles. A dramatic decrease in the overvoltage of H2O2 was observed with improved sensitivity, which makes the modified electrodes of great promise for oxidase-based amperometric biosensors. The biosensor responded to H2O2 in the linear range from 1.2×106 mol/L to 9.8×103 mol/L with a detection limit of 1.1×108 mol/L. Moreover, the obtained biosensor exhibited good accuracy and high sensitivity.

Keywords

AgNP Hydrogen Peroxide Cycteamamin Biosensor Cyclic Voltammetry

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