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Received June 2, 2011
Accepted June 26, 2011
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Physicochemical properties and bioactivity of brown seaweed fucoidan prepared by ultra high pressure-assisted enzyme treatment
Department of Food and Biotechnology and Natural Choice Co., Ltd., Hoseo University, Asan 336-795, Korea 1Department of Human Nutrition and Food Science, Chungwoon University, Hongseong 350-701, Korea
hjchae@hoseo.edu
Korean Journal of Chemical Engineering, February 2012, 29(2), 221-227(7), 10.1007/s11814-011-0165-7
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Abstract
The effects of ultra-high pressure (UHP, 100 MPa, 40, 24 h) processing and enzyme treatment on the physicochemical properties and anticoagulant activity of fucoidan obtained from Undaria pinnatifida sporophyll (UPS) were investigated. Crude UPS fucoidan (FUPS) with an average MW of 877 kDa was slightly depolymerized by UHPassisted Tunicase treatment, yielding fucoidan with a lower MW (600-800 kDa). UHP-enzyme treatment decreased the sulfate, fucose and galactose contents of FUPS but increased its glucose content. From FT-IR spectrum, UHP-enzyme treatment was found not to cause a structural change on S=O and C-O-S. After UHP-enzyme treatment, the sulfate content and average MW of FUPS decreased with the increase of Tunicase concentration. It was found that among the UHP-enzyme-treated fucoidans, FUPS-T0.3-U (Tunicase 0.3% treatment+UHP treatment) possessed the highest anticoagulant activity. FUPS-T0.3-U appeared to inhibit blood coagulation via intrinsic pathway. With the increase of sulfate content in FUPS, activated partial thromboplastin time (APTT) and thrombin time (TT) showed a tendency of increase. APTT and TT had the highest values in FUPS-T0.3-U in which the sulfate concentration was 24%, but on the contrary decreased at a sulfate concentration of above 24%. This result indicates that there is an optimum sulfate concentration for the anticoagulant activity of fucoidan. Consequently, UHP-assisted enzymatic treatment was found to be helpful for the improvement of anticoagulant activities of fucoidan.
Keywords
References
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Jack H, Valentin F, Heparin Circul., 89, 2205 (1994)
Qiu X, Amarasekara A, Doctor V, Carbohydr. Polym., 63, 224 (2006)
Usui T, Asari K, Mizuno T, Agric. Biol. Chem., 44, 1965 (1980)
Kim YM, Kim DS, Choi YS, Korean J. Food Sci. Technol., 36, 1008 (2006)
Furusawa E, Furusawa S, Oncology., 46, 343 (1989)
Han JG, Syed AQ, Kwon M, Ha JH, Lee HY, J. Biotechnol., 136, S571 (2008)
Aisa Y, Miyakawa Y, Nakazato T, Shibata H, Saito K, Ikeda Y, Kizaki M, Am. J. Hematol., 78, 7 (2005)
Raghavendran HRB, Srinivasan P, Rekha S, Inter. Immunopharm., 11, 157 (2011)
Cooper R, Dragar C, Elliot K, Fitton JH, Godwin J, Thompson K, BMC Complement. Altern. Med., 2, 11 (2002)
Ponce NMA, Pujol CA, Damonte EB, Flores ML, Stortz CA, Carbohydr. Res., 338, 153 (2003)
Daniel R, Berteau O, Chevolot L, Varenne A, Gareil P, Goasdoue N, Eur. J. Biochem., 268, 5617 (2001)
Veena CK, Josephine A, Preetha SP, Varalakshmi P, Food Chem., 100, 1552 (2007)
Jing W, Li L, Quanbin Z, Zhongshan Z, Huimin Q, Pengcheng L, Food Chem., 114, 1285 (2009)
Alexander OC, Anne D, Howard RM, Stuart MH, Roy AM, Alexander SS, Nikolay EN, Elena AK, Anatoli IU, Carbohydr.Res., 320, 108 (1999)
Koo JG, Choi YS, Kwak JK, J. Korean Fish. Soc., 34, 515 (2001)
Kloareg B, Demarty M, Mabeau S, Inter. J. Biol. Macromol., 8, 380 (1986)
Patankar MS, Oehninger S, Barnett T, Williams RL, Clark GF, J. Biol. Chem., 268, 21770 (1993)
Bae JS, Lee JS, Kim YS, Sim WJ, Lee H, Chun JY, Park KP, Korean Chem. Eng. Res., 46(5), 886 (2008)
Bohn JA, Bemiller JN, Carbohydr. Polym., 28, 3 (1995)
Grauel V, Kloareg B, Mabeau S, Durand P, Jozefonvicz J, Biomaterials., 10, 363 (1989)
Nishino S, Mignot E, Benson KL, Zarcone VP, Psychiatry Res., 78, 141 (1998)
Corrales M, Toepfl S, Butz P, Knorr D, Tauscher B, Inno. Food Sci. Emerg. Technol., 9, 85 (2008)
Prasad KN, Yang B, Shi J, Yu C, Zhao M, Xue S, Jiang Y, J. Pharm. Biom., 51, 471 (2010)
Zhang S, Junjie Z, Changzhen W, Int. J. Pharm., 278, 471 (2004)
Alain R, Nadon F, Seguin C, Payment P, J. Virogical Meth., 16, 209 (1996)
Koyanagi S, Tanigawa N, Nakagawa H, Soeda S, Shimeno H, Biochem. Pharmacol., 65, 173 (2003)
Furukawa SI, Fujikawa T, Koga D, Ide A, Jap. Soc. Fish.Sci., 58, 1499 (1992)
Schaeffer DJ, Kryolv VS, Ecotoxicol. Environ. Safety., 45, 208 (2000)
Pielesz A, Biniaoe W, Paluch J, Carbohydr. Res., In Press
Lionel H, Legrand E, Rastogi N, FEMS Immunol. Medical Microbiol., 23, 37 (1999)
Daniel R, Berteau O, Jozefonvicz J, Goasdoue N, Carbohydr.Res., 322, 291 (1999)
Kim SJ, Kim YS, Jung ST, Korea Patent 0,047,488 (2004)
Ahmed J, Ramaswamy HS, Stewart. Postharvest Rev., 1, 1 (2006)
Dornenburg H, Knoor D, Food Biotechnol., 48, 735 (1993)
Zhang SQ, Bi HM, Liu CJ, Sep. Purif. Technol., 57(2), 277 (2007)
Zhang S, Chen R, Wu H, Wang C, J. Pharm. Biomed. Anal., 41, 57 (2006)
Pan Y, Wang K, Huang S, H. Wang H, Mu X, He C, Ji X, Zhang J, Huang F, Food Chem., 106, 1264 (2008)
Yang B, Zhao M, Shi J, Yang N, Jiang Y, Food Chem., 106, 685 (2008)
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F, Anal. Chem., 28, 350 (1956)
Dodgson KS, Price RG, J. Biochem., 84, 106 (1962)
Lee JS, Ra KS, Son HS, Korean J. Food Sci. Technol., 27, 860 (1996)
Koo JG, Korean J. Fish. Soc., 30, 128 (1997)
Park K, Ph.D. Thesis, Hoseo University (2008)
Bae JS, Lee JS, Kim YS, Sim WJ, Lee H, Chun JH, Park KP, Korean Chem. Eng. Res., 46, 886 (2007)
Mori H, Kamei H, Nishide H, Nisizawa K, Proc. Seaweed Symp., 10, 109 (1982)
Svetlana NK, Irina YB, Yulia VB, Viktor IE, Natalia YK, Konstantin VG, Valeri BK, Valeri AR, Tatyana NZ, Carbohydr.Res., 344, 191 (2009)
Yuya K, Takao O, Comp. Biochem. Physiol., 10, 1016 (2009)
Percival E, Jara MFV, Weigel H, Carbohydr. Res., 125, 283 (1987)
Dobashi K, Nishino T, Fufihara M, Nagumo T, Carbohydr.Res., 194, 315 (1989)
Nishino T, Aizu Y, Nagumo T, Thrombosis Res., 64, 723 (1991)
Fujihara M, Lizima N, Yamanoto I, Nagumo T, Carbohydr.Res., 125, 97 (1984)
Lloyd AG, Dodgson KS, Price PG, Rose FA, Biochem.Biophys. Acta., 46, 108 (1961)
Kim YS, Biochem. Mol. Biol. News., 10, 36 (1990)
Lee HS, Kweon MH, Lim WJ, Sung HJ, Yang HC, Korean J. Food Sci. Technol., 29, 369 (1997)
Lee HS, Jin SH, Kim HS, Ryu BH, Korean J. Food Sci., 27, 716 (1995)
Church FC, Meade JB, Treanor RE, Whinna H, J. Biol.Chem., 264, 3618 (1989)
Mauray S, Sternberg C, Theveniaux J, Millet J, Sinquin C, Thromb. Haemost., 74, 1280 (1995)
Colliec S, Fihser AM, Tapon-Bretaudiere J, Boisson C, Durand P, Jozefonvicz J, Thromb. Res., 64, 143 (1991)
Nishino T, Nagumo T, Carbohydr. Res., 214, 193 (1991)
Haroun-Bouhedja F, Ellouali M, Sinquin C, Boisson-Vidal C, Thromb. Res., 100, 453 (2000)
Yuan H, Zhang W, Li X, Li N, Gao X, Carbohydr. Res., 340, 685 (2005)
Jack H, Valentin F, Heparin Circul., 89, 2205 (1994)
Qiu X, Amarasekara A, Doctor V, Carbohydr. Polym., 63, 224 (2006)
