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Received June 15, 2011
Accepted July 19, 2011
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Characterization of oil including astaxanthin extracted from krill (Euphausia superba) using supercritical carbon dioxide and organic solvent as comparative method

International Program of Fisheries Sciences, Faculty of Fisheries Sciences, Pukyong National University, 599-1, Daeyeon-3dong, Nam-gu, Busan 608-737, Korea 1Department of Food Science and Technology, Faculty of Fisheries Sciences, Pukyong National University, 599-1, Daeyeon-3dong, Nam-gu, Busan 608-737, Korea
bschun@pknu.ac.kr
Korean Journal of Chemical Engineering, March 2012, 29(3), 329-336(8), 10.1007/s11814-011-0186-2
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Abstract

Krill oil including astaxanthin was extracted using supercritical CO2 and hexane. The effects of different parameters such as pressure (15 to 25MPa), temperature (35 to 45 ℃), and extraction time, were investigated. The flow rate of CO2 (22 gmin.1) was constant for the entire extraction period of 2.5 h. The maximum oil yield was found at higher extraction temperature and pressure. The oil obtained by SC-CO2 extraction contained a high percentage of polyunsaturated fatty acids, especially EPA and DHA. The acidity and peroxide value of krill oil obtained by SC-CO2_x000D_ extraction were lower than that of the oil obtained by hexane. The SC-CO2 extracted oil showed more stability than the oil obtained by hexane extraction. The amount of astaxanthin in krill oil was determined by HPLC and compared at different extraction conditions. The maximum yield of astaxanthin was found in krill oil extracted at 25 MPa and 45 ℃.

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